Papers by Keyword: Proliferation

Abstract: Polychlorinated biphenyls and their hydroxylated metabolites become more and more concerned for their endocrine disruption. At the presented study, experiment was designed to obtain the disruption effect of PCB101 and its hydroxylated metabolites . The proliferation of MCF-7 human breast cancer cells was detected at different exposed concentration and time period. MCF-7 cells were cultured with the concentration of PCB101 and twor of its hydroxylated metabolites at 5×10^-95×10^-85×10^-75×10^-6mol/L. And the concentrations of 17β-Estradiol (E2) were2×10^-92×10^-82×10^-72×10^-6mol/L respectively. And the proliferation rate was detected after 24h,48h, 72h, 96h exposure.Ethanol was used as solvent control.The results indicated that PCB101 and its hydroxylated metabolites had no effect on MCF-7 cell proliferation at designed concentrations and exposure time.

Abstract: To evaluate the male reproductive toxicity of PFOS on mammal animals at cellular level, mouse leydig cells were isolated from healthy mouse testis tissue and cultured in vitro. Adherent cells were treated with a serial concentration of PFOS for 4 more days of culture. Proliferation and DNA damage of the cells were analyzed by CCK assay and SCGE assay respectively. Forty-eight hours of treating with PFOS≧25μg/mL all inhibited the proliferation of the cells (p<0.05). PFOS seemed not to change the time for the cells to reach platform phase. DNA damage was also observed in the groups treated with PFOS dependent on dose and exposure time. The highest DNA damage level was averagely 17 cells per well in 96-well plates, which occurred to 62.5μg/mL group at 72h.

Abstract: Many studies have focused on the fabrication of Cu nanowires, because of their potential applications in diverse fields such as nanoelectronics and gene delivery. In this study, we successfully fabricated Cu nanowires by electrochemical deposition method. Cu nanowires was synthesized by electroplating from a 0.4M CuSO₄ bath, and the current was 0.005A. The length of nanowires can be controlled via deposition time and current. After depositing for 60 minutes, the length of the nanowires is approximately 10 µm. Serum coated and non-coated nanowires with the concentration of 10², 10³, 10⁴ and 10⁵ were added to 96-well cell culture plate in which mesenchymal stem cells were pre-seeded. After incubating for 3 days, cytotoxicity of nanowires was measured with MTT assay method. Results indicated that cytotoxicity increased as the increase of nanowires concentration. Serum coated nanowires had higher cell viability as compared with the non-coated group.

Abstract: Abstract Objective To testify the special cytotoxicity of TGF-alpha-SAP on proliferating vascular smooth muscle cells and endothelial cells. Methods Conjugation of saporin to TGF-alpha was accomplished after derivatization of saporin and TGF-alpha with N-succinimidyl-3 (2-pyridyldithio) proprionate and final purification of the conjugate was achieved within Eppendorf Centrifugal Filter Cytotoxicity assays were measured by cell count. The studies of influence of TGF-alpha-SAP on values of Thymidine and leucine incorporation into SMCs and ECs were measured by 3H-thymidine uptake and 3H-leucine uptake, respectively. and receptor competition studies of TGF-alpha-SAP are measured by adding excess TGF-alpha in SMCs exposed for TGF-SAP. Results Cytotoxicity assays testified TGF-alpha-SAP conjugate could inhibit remarkably proliferation of SMCs in culture. The values of thymidine of TGF-alpha-SAP group (10-9M and 10-7M) in comparison significantly decreased to 60.9% and 56.0% of the control group respectively, suggesting that cellular DNA synthesis obviously decreased as TGF-alpha-SAP was added. But Saporin did not affect cellular DNA synthesis at higher level. The rate of 3H-leucine incorporation of TGF-alpha-SAP group significantly decreased to 47.3% of the control group, suggesting that SMCs protein synthesis obviously decreased as TGF-alpha-SAP was added. But TGF-alpha-SAP at the same level did not affect DNA synthesis and protein synthesis of ECs compared with the control group. Conclusion The results indicated that TGF-alpha-SAP possesses the more effective cytotoxicity than Saporin and the more special citotoxicity on proliferating vascular smooth muscle cells than on proliferating endothelial cells. Key words：cytotoxicity;selective; Drug-eluting stents;proliferation;saporin

Abstract: In the structure of the product modeling design, there is a kind of Proliferated form that is shown as form in quantity proliferation, is also proliferation in the form of all kinds. Proliferating form endowed modeling with new vitality and the urge to multiply. Proliferating form includes the structural proliferation, derivative proliferation, chaotic proliferation, heterogeneous proliferation and repetitive proliferation

Abstract: Andrographolide (1) is a primary constituent of Andrographis paniculata (Burm. f.) Nees which is a Chinese herbal medicine possesses extensive pharmacological effects. In this paper, the effect of compound 1 and some 15-alkylidene substituted derivatives of andrographolide (2-13) on murine splenic T lymphocyte proliferation reaction were appraised using MTT assay. The results showed that most of 15-alkylidene substituted andrographolides appraised could exert potent immunosuppressant effects. Especially compound 5, the most potent among all the tested compounds at concentrations with no obvious cytotoxicity, significantly inhibited the proliferation of murine splenic T lymphocytes activated by Con A in a dose-dependent manner (1.56μmol/l-12.50μmol/l), by the most inhibition of 55.8%. Findings implied that suitable 15-alkylidene substituted andrographolides might possess some better pharmacology activity than 1.

Abstract: This study presents a novel synthetic approach for the preparation of cell attachable microfibers in microfluidic system. The synthesis is performed by multiphase laminar flows with spontaneous formation of carbon dioxide bubbles resulting asymmetrically porous PU microfiber. The fabricated Janus microfiber provides two distinctive properties: one is porous region to promote the cellular adhesion and the other is nonporous region rendering mechanical strength of the scaffold. The Janus microfibers show dramatic improvement of cell adhesion, proliferation, and viability over a culture period. The Janus microfiber can be used for not only alternative 2D cell culture plate but also as novel 3D scaffold for tissue engineering wihtout any need of elegant surface modification for enhancing cell adhesions.

Abstract: Effects of Daidzein or Genistein on the proliferation and antioxidation of mammary epithelial cells of dairy cow were investigated in vitro. 10 groups were assigned including blank control, estradiol (E₂) group(10 ng/mL), different concentrations of Daidzein (1, 10, 100, 1 000 ng/mL) and Genistein (1, 10, 100, 1 000 ng/mL) groups. The MMT method was used to determine the proliferation effect of Genistein or Daidzein, and the results showed that Genistein at the concentration of 10 and 100 ng/mL, and Daidzein at the concentration of 100 and 1000 ng/mL significantly improved dairy cow mammary epithelial cell proliferation (P<0.05) , while significantly weaker than E₂ group(P<0.05). In the antioxidation experiment, the T-SOD and GSH-PX activity, MDA and NO content of the mammary epithelial cells at the logarithmic growth phase treated with Daidzein or Genistein for 24 h were measured and the results showed that 100, 1000 ng/mL Daidzein, and 100, 1000 ng/ mL Genistein significantly increased the T-SOD activities and decreased MDA content (P<0.05).1 000 ng/ mL Daidzein and 100, 1 000 ng/mL Genistein significantly increased the GSH-PX activites (P<0.05). The results showed that proper levels of daidzein and genistein can improv the proliferation and antioxidation function of mammary epithelial cell of dairy cows.

Abstract: Sertoli cells are an important part of the male reproductive system. Except the function of promoting spermatogenesis such as supporting, providing nutrition and secreting, it also can maintain the immune privilege of testis through the expression of a special surface molecule named FasL. This feature aviods the immune response triggered by self antigen (germ cells) contacting with the body, which can be used to provide immune privilege environment for transplanted cells in areas other than the testis. In this article, we purified mouse sertoli cells, through adding different concentrations of IGF-I and IGF-I receptor inhibitor PPP, we study the role of IGF-I in the proliferation of sertoli cells. The results demonstrated that 30ng/mL IGF-I can promote the proliferation of sertoli cells while 2nmol/L PPP obviously inhibited the proliferation of sertoli cells. This result has a certain significance to obtain the high activity sertoli cells and increase the success rate of xenotransplantation.

Abstract: Tetrandrine can inhibit the proliferation and collagen synthesis of fibroblasts in lung and liver tissue confirmed by a series of clinical research. In this chapter, we investigated the effect of Tetrandrine on the proliferation of human dermal fibroblasts derived from hypertrophic scars. The dermal fibroblasts were isolated from human hypertrophic scar tissues and cultured in vitro. Tetrandrine with different concentration were added to culture medium respectively. The proliferative activities were determined. The result show that when the concentration of added Tetrandrine increased from 5μg/ml to 80μg/ml, the proliferative activities of cultured dermal fibroblasts were decreased gradually in dose-dependent manner. It conclusions that Tetrandrine can obviously inhibit the proliferation of human dermal fibroblasts derived from hypertrophic scars.