Papers by Keyword: Saliva

Abstract: Two AISI (316,304) stainless steel alloys were utilized in this work to enhance the surface properties and corrosion inhibiters with Al₂O₃ nanoparticles which was generated via pulse laser deposition (PLD). Alumina as biomaterial for dental purposes was prepared using the co-precipitation process and analysis by X-Ray diffraction. Alumina has drawn a lot of interest and is historically well-accepted .SEMs, and EDS, been used to describe researchers have studied the coating morphology. Roughness and Vickers hardness were utilized to quantify the surface modifications as part of an investigation into corrosion prevention employing mechanical properties submerged in saliva with a PH (5.6). When compared to bare AISI (316,304) stainless steel, all samples exhibit greater corrosion resistance.

Abstract: Abstract. To analyze saliva and tongue coating pH in healthy patients with physiological halitosis before and after usage of 5 different types of mouthwash available in Malaysia. A quasi-experimental study was conducted among preclinical IIUM dental students aged 18 to 21 years old. Organoleptic evaluation was conducted by the operator to rank their organoleptic score followed by self-assessment by the participants. Saliva and tongue coating pH we measured respectively beforehand, immediately after and 30 minutes after rinsing with 5 different mouthwashes using a digital pH meter and pH strip indicator respectively; 100 participants were assigned into 5 groups, with 20 samples for each brand. Intraclass correlation coefficient between examiners ranged from 0.76-1.0. Data were analyzed statistically by repeated measure ANOVA test for each brand of mouthwash. Multivariate Analysis of Covariance (MANCOVA) + test was also used to statistically analyze different pH trends among the five brands of mouthwashes. Significant increase of salivary pH was observed after using MW2 (p<0.001) and MW4 (p=0.015). Significant reduction in salivary pH was observed when using MW5 (p=0.001). For tongue coating pH, significant reduction was observed after using MW2 (p<0.0010), MW3 (p=0.012), and MW4 (p=0.001). However, there was a significant increase in tongue coating pH after using MW5 (p=0.0125). Rinsing with mouthwashes MW2, MW3 and MW4 decreased tongue coating pH, which leads to reduced physiological halitosis

Abstract: In this research studied on the conjugation of antibodies on gold nanoparticles (GNPs) and the optimization in term of antibody concentration and albumin blocking concentration. Gold nanoparticleswith diameter size of ~20 nm were synthesized via chemical reduction of HAuCl₄ with trisodium citrate as reducing and stabilizing agent. Gold nanoparticles and anti-A antibody at various concentrations were mixed and nonspecific binding sites were blocked by using bovine serum albumin (BSA) at various concentrations. The antibody conjugated gold nanoparticles were tested by coagulation with 1% sodium chloride salt solution to obtain the optimal antibody concentration. BSA 50, 100 and 200 μg/mL were added in solution after antibody covered on gold nanoparticles. The solutions were characterized by measuring absorbance spectra. From the results found that the optimal concentration of antibodies and BSA blocking were 45 μg/mL and 50 μg/mL, respectively. The anti-A conjugated gold nanoparticles were tested with saliva of blood group A sample to monitor the specific interaction between antibody on gold surface and antigen in saliva via the change of absorbance spectra.

Abstract: The saliva pregnancy test apparatus is dependent on manual operation to obtain results by observing the saliva crystallization image with complex operation, how to recognize the saliva crystallization image quickly and accurately has become an important research topic. In this paper, an image recognition method on the crystallization of saliva is proposed. Firstly, gray processing on the original image, improved Otsu method is used to select the threshold and binaryzation. Then the proportion of black and white pixels is calculated to identify saliva crystal images with the percentage values. The results show that the method is simple and treatment results are accurate.

Abstract: : The pellicle on oral surfaces represents a central interface for the formation of biofilms. Among other things it causes the first adsorption of bacteria. The dynamics of pellicle formation, on tooth surfaces and the influence of oral therapeutics on the pellicle structure are fairly unknown. With the method of surface plasmon resonance (SPR), the formation of salivary pellicle structures on hydroxylapatite (HAP) surfaces covering a very thin (~50nm) layer gold on a glass prism was recorded in real time without labeling or destruction. As pellicle forming substrates natural pooled human saliva (NS) and artificial saliva (AS) were used. To simulate the influence of therapeutic additives on the dynamic of the pellicle forming process, a chlorhexidine preparate (Chlorhexamed Fluid® CHX) on two different concentrations was selected. The binding behavior of a NS and a preparation in terms of an AS were compared. The layer was largely stable against rinsing with buffer. The application of CHX preparations in two different concentrations as an example of an oral therapeutic additive revealed a complex dynamic of adsorption. CHX did not lead to any visible destruction of the pellicle. The introduced method is an excellent tool to illustrate the dynamic effects of pellicle formation or pellicle reorganization by measuring the increase or decrease of the SPR signal in real time.

Abstract: Self-assembled niobium oxide microcones produced by potentiostatic anodization with varied NaF content (between 100 and 250 mg) in an HF electrolyte are shown to nucleate mineral when immersed in supersaturated solutions emulating mineral content in saliva and blood. The most extensive mineral coverage in 100 mL of 2.5 wt. % HF electrolyte occurs when NaF content is about 100 mg with substantial mineral formation occurring within 24 hours. Higher salt content apparently alters the conditions favoring mineral nucleation by generating smaller nucleation centers that ultimately diminish the extent of mineral coverage. Additionally, nucleation kinetics and morphological contrasts between mineral formed from saliva and blood is briefly discussed in terms of the relative degree of supersaturation with respect to hydroxyapatite. Finally, we show that the integrity of the microcone shape is not critical for mineral nucleation, an observation that builds on our prior hypothesis by promoting the importance of self-assembly and crystal formation. Based on these results, we demonstrate the influence of NaF and stress the role of the self-organization process in producing effective mineral nucleation sites.