Papers by Keyword: WST-1

Abstract: Cytotoxicity test was essential for the pre-clinical evaluation of bioceramics. Proliferation assays such as MTT, XTT and WST-1 were commonly used for measuring biocompatibility. WST-1 was more convenient than MTT because of its water-solubility and storage condition. The calcium phosphate glass and β-TCP have been used for bone substitute, and some magnetic ferrites have been used for hyperthermic treatment. L929, mouse fibroblast cell, was the representative cell-line for in vitro biocompatibility test. The extracts of test samples were prepared by ISO10993-12:2002. The biocompatibilities of the extracts were measured by MTT and WST-1 assay and their pH were measured with pH meter. The cellular survival rate of CPG was the lowest and the results of the WST-1 test showed results similar to those of the MTT test. Thus, proliferation assays using WST-1 may be conveniently and routinely applicable to pre-clinical evaluation of bioceramics.

Abstract: The purpose of this study was to evaluate the cytotoxicity of alginate-encapsulting ferrite particles in vitro. Various ferrite particles such as Ba-ferrite, Sr-ferrite, Co-ferrite, Co/Ni-ferrite were prepared by sol-gel process. Ferrite particles were encapsulated via calcium alginate process with different alginate contents ranged from 10 to 100 wt%. Mouse-fibroblastic NCTC L-929 cells were cultured in RPMI-1640 medium with 10% fetal bovine serum. The alginate-encapsulating ferrites were extracted in 5 ml of distilled water under pH 6.5 at 121°C for 1 h in accordance with ISO 10993-12. In vitro cytotoxicity was evaluated by WST-1. The results of this study indicated that the alginate-encapsulting ferrite particles affected cell viability by increasing alginate contents. Especially, alginate-encapsulating process were enhanced cell viability of ferrites such as Sr-ferrite, Co/Ni-ferrite, and Ba-Ferrite when alginate content was 10 wt%.