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Online since: October 2011
Authors: Yan Hong Chang, Hui Luo, Yao Zhou, Lian Xiong, Lin Zhi Liu
Rapid Gene Cloning, Overexpression and Characterization of a Thermophilic Catalase in E. coli
1Hui Luo*, 1Yao Zhou, 2Yanhong Chang, 2Lian Xiong, 2Linzhi Liu
1Department of Biological Science and Engineering, University of Science and Technology Beijing, Beijing, P.R.China
2Department of Environmental Engineering, University of Science and Technology Beijing, Beijing, P.R.China
1e-mail: luohui@ustb.edu.cn, 2e-mail: yhchang0512@yahoo.com.cn
Keywords: Catalase; thermophilic; overexpression; enzyme properties; screening
Abstract.
Materials and Methods A.
Fang, et al, “Construction of recombinant thermostable catalase engineering strain and optimization of its fermentation condition,” Journal of Food Science and Biotechnology, vol. 25, no. 2, pp. 74-78, 2006
Chen, “Thermo-alkali-stable catalase from thermoascus aurantiacus and its potential use in textile bleaching process,” Chinese Journal of Biotechnology, vol. 20, no. 3, pp. 423-428, May 2004
Negoro, “Cloning nucleotide sequence and exepression in E.coli of the Bacillus stearothermophilus peroxidase gene,” Journal of Bacteriology, vol. 171, no. 9, pp. 4871-4875, September 1989
Materials and Methods A.
Fang, et al, “Construction of recombinant thermostable catalase engineering strain and optimization of its fermentation condition,” Journal of Food Science and Biotechnology, vol. 25, no. 2, pp. 74-78, 2006
Chen, “Thermo-alkali-stable catalase from thermoascus aurantiacus and its potential use in textile bleaching process,” Chinese Journal of Biotechnology, vol. 20, no. 3, pp. 423-428, May 2004
Negoro, “Cloning nucleotide sequence and exepression in E.coli of the Bacillus stearothermophilus peroxidase gene,” Journal of Bacteriology, vol. 171, no. 9, pp. 4871-4875, September 1989
Online since: April 2012
Authors: Xiao Gang Wang, Jin Chen
Influence of surface modification on Si3N4 powder fluidity
Jin Chen *, Xiaogang Wang
Faculty of Materials science, Xi’an University of Science and Technology, Xi’an, 710070,
Key words:Silicon nitride; surface modification; organic coating modification;
Abstract: The effect of organic modification on the surface of silicon nitride powders has been investigated.
All these superior characters has arouse a lot of interest by materials researchers[2].
When bulk granular materials are poured onto a horizontal surface, a conical pile will form.
Acknowledgments This work was supported by the Scientific Research Program Funded by Shaanxi Provincial Education Commission(Program NO. 2010JK676) References [1] Zweben, C., Advances in composite materials for thermal management in electronic packaging.
Journal of the European Ceramic Society.2004:3479-3484
All these superior characters has arouse a lot of interest by materials researchers[2].
When bulk granular materials are poured onto a horizontal surface, a conical pile will form.
Acknowledgments This work was supported by the Scientific Research Program Funded by Shaanxi Provincial Education Commission(Program NO. 2010JK676) References [1] Zweben, C., Advances in composite materials for thermal management in electronic packaging.
Journal of the European Ceramic Society.2004:3479-3484
Online since: September 2011
Authors: Chang Rong Liao, Qiong Liu, Dan Xia Zhao, Lei Xie
By 2008, Liu Jing-quan et al.[4] from Shanghai Jiaotong University had presented a generator array with Cantilever piezoelectric materials, which was able to control the frequency bandwidth to improve conversion efficiency and to generate a power of 3.98 μW as well as a direct voltage of 3.93V.
During 2007 and 2008, a micro-vibration generator with magnetostrictive materials (Metglas 2605SC) had been proposed by Wang and Yuan[4,5] to be used in harvesting energy for wireless sensor networks, which provided an electric power of 576μW, appearing far higher than one with piezoelectric materials on the same conditions.
Microelectronics Journal, 39 (2008)802–806 [5] Wang L and Yuan F G. 2007 Energy Harvesting by magnetostrictive material (MsM) for powering wireless sensors in SHM 14th Int.
SPIE Smart Structures and Materials & NDE and Health Monitoring, [6] Wang L and Yuan F G.
Vibration Energy harvesting by magnetostrictive material [J].
During 2007 and 2008, a micro-vibration generator with magnetostrictive materials (Metglas 2605SC) had been proposed by Wang and Yuan[4,5] to be used in harvesting energy for wireless sensor networks, which provided an electric power of 576μW, appearing far higher than one with piezoelectric materials on the same conditions.
Microelectronics Journal, 39 (2008)802–806 [5] Wang L and Yuan F G. 2007 Energy Harvesting by magnetostrictive material (MsM) for powering wireless sensors in SHM 14th Int.
SPIE Smart Structures and Materials & NDE and Health Monitoring, [6] Wang L and Yuan F G.
Vibration Energy harvesting by magnetostrictive material [J].
Online since: September 2014
Authors: Yuriy N. Yurjev, Dmitrii V Sidelev, Danil A. Zaitcev, Olga S. Tupikova
Surdu-Bob, Diamond Film Nano-Abrasives Obtained by Anodic Arc, Optoelectronics and Advanced Materials 3 (2009) 1207-1209
Fukuda, Tribological Characteristics of DLC Coatings in Vacuum under Sliding Contact, Journal of Surface Engineered Materials and Advanced Technology 2 (2012) 22-27
Mercado, Diamond-Like Carbon and Ceramic Materials as Protective Coatings Grown by Pulsed Laser Deposition, Aachen, 2004
Wojtalik, Optimization of the Deposition Parameters of DLC Coatings with the MCVA Method, Archives of Materials Science and Engineering 54 (2012) 60-67
Baroch, Discharge in Dual Magnetron Sputtering System, IEEE Transactions on Plasma Science 33 (2005) 338-339
Fukuda, Tribological Characteristics of DLC Coatings in Vacuum under Sliding Contact, Journal of Surface Engineered Materials and Advanced Technology 2 (2012) 22-27
Mercado, Diamond-Like Carbon and Ceramic Materials as Protective Coatings Grown by Pulsed Laser Deposition, Aachen, 2004
Wojtalik, Optimization of the Deposition Parameters of DLC Coatings with the MCVA Method, Archives of Materials Science and Engineering 54 (2012) 60-67
Baroch, Discharge in Dual Magnetron Sputtering System, IEEE Transactions on Plasma Science 33 (2005) 338-339
Online since: January 2005
Authors: M. Pan, Run Zhang Yuan, Shi Chun Mu
Introduction
Porous solid materials have created extensive research activity because of low working pressure,
light weight and changeable form in hydrogen storage.
Subsequently, Long et al. [2] explored a new type of microporous metal coordination materials (MMOMs) with pore dimension comparable to length scale of the molecular diameter (0.289nm) of hydrogen.
The first two samples, sepiolite and palygorskite, had obvious hydrogen uptake platforms: the first may indicate the hydrogen adsorbing on the outer surface area of materials up to saturation, while the second shows that hydrogen may enter into channels and achieve saturation.
Science Vol.300 (2003), P.1127 [2] L.
Aoufi, et al., Journal of Chemical Physics Vol.112 (2000) P, 5991 [7] J.
Subsequently, Long et al. [2] explored a new type of microporous metal coordination materials (MMOMs) with pore dimension comparable to length scale of the molecular diameter (0.289nm) of hydrogen.
The first two samples, sepiolite and palygorskite, had obvious hydrogen uptake platforms: the first may indicate the hydrogen adsorbing on the outer surface area of materials up to saturation, while the second shows that hydrogen may enter into channels and achieve saturation.
Science Vol.300 (2003), P.1127 [2] L.
Aoufi, et al., Journal of Chemical Physics Vol.112 (2000) P, 5991 [7] J.
Online since: March 2013
Authors: Ru Heng Wang, Xiao Liu, Rui Liu, Mu Yi Hou
Then trim dry timber material as the size of groove and splice it in the groove.When the Surrounding wood of beam or column all need to trim,it must add 2 or 3 iron hoops to remain stable
(5) FRP reinforcement.Take FRP materials to reinforce timberwork frame.The common materials such as GFRP,CFRP and BFRP have the advantages of light weight, high strength, corrosion resistance, easy cutting, save artificial.FRP materials not olny improve beam’s and column’s carrying capacity, stiffness and ductility but also enhance its durability.
Conclusions Due to its special structure and excellent properties of materials, timberwork building is endowed with excellent seismic capability.But long neglected and in disrepair make the aseismatic function of these buildings recede seriously.In order to protect historical buildings and ensure people's life and property safety,we need analyse the causes of damage and provide the objective of earthquake-resistance to provide basis and method in timberwork building reinforcement.
Acknowledgements This work was financially supported by Fund about the Tibet autonomous region of science and technology plan projects (Tibet 2012-01-11).
Study on the seismic performance of Chinese ancient timber structure [J]. northwestern seismological journal, 2008, 30, (3):239-244.
(5) FRP reinforcement.Take FRP materials to reinforce timberwork frame.The common materials such as GFRP,CFRP and BFRP have the advantages of light weight, high strength, corrosion resistance, easy cutting, save artificial.FRP materials not olny improve beam’s and column’s carrying capacity, stiffness and ductility but also enhance its durability.
Conclusions Due to its special structure and excellent properties of materials, timberwork building is endowed with excellent seismic capability.But long neglected and in disrepair make the aseismatic function of these buildings recede seriously.In order to protect historical buildings and ensure people's life and property safety,we need analyse the causes of damage and provide the objective of earthquake-resistance to provide basis and method in timberwork building reinforcement.
Acknowledgements This work was financially supported by Fund about the Tibet autonomous region of science and technology plan projects (Tibet 2012-01-11).
Study on the seismic performance of Chinese ancient timber structure [J]. northwestern seismological journal, 2008, 30, (3):239-244.
Online since: January 2013
Authors: Yu Xiang Li, Guang Bin Zhang, Xing Shun Liu, Zhen Bo Gao, Xin Liu
Students can view multimedia courseware and educational videos and other teaching materials through websites to actively study the theoretical knowledge in software engineering by themselves.
This function module mainly provides students with knowledge-related resources, such as: relevant international journals, books and Web resources, assists learners in keeping abreast of developments in international and domestic disciplines and finding relevant information.
Through the above analysis, we can know the entities mainly got involved in the system include: users, teachers, assignments, examination questions, teaching resources and so on, and relationship among entities is complex, the analysis is as follows: The Entities Got Involved in Management of Learning Materials Include: Users, Teachers and Learning Materials.
Properties of learning materials include serial number, name, type, storage address and updated time.
E-R diagram on management of study materials The Entities Got Involved in Management of Announcement Information Include: Users, Teachers and Announcement Information.
This function module mainly provides students with knowledge-related resources, such as: relevant international journals, books and Web resources, assists learners in keeping abreast of developments in international and domestic disciplines and finding relevant information.
Through the above analysis, we can know the entities mainly got involved in the system include: users, teachers, assignments, examination questions, teaching resources and so on, and relationship among entities is complex, the analysis is as follows: The Entities Got Involved in Management of Learning Materials Include: Users, Teachers and Learning Materials.
Properties of learning materials include serial number, name, type, storage address and updated time.
E-R diagram on management of study materials The Entities Got Involved in Management of Announcement Information Include: Users, Teachers and Announcement Information.
Online since: July 2011
Authors: Xian Leng, Lu Hai Li, Wen Bo Li, Xu Wei Hu, Wei Wei Li, Hua Fang
The color depth of materials refers to the light waves' position in the spectrum.For dye chemical,people have the effet by increased absorption wavelength called bathochromic effect, conversely, the effet by reduce absorption wavelength called hypsochromic effect[1].
Acknowledgement The study is financially supported by Key Project Funding of Beijing Municipal Education & Beijing natural science foundation Committee (KZ200910015001) and Funding Project for Academic Human Resources Development in Institutions of Higher learning under the jurisdiction of Beijing Municipality PHR(IHLB) (PXM2009-014223-077341);(PHR200909124); Opening investigation subject of Beijing area key lab of pringting & packaging material and technology (OISBKPP)(KF201010) References [1]Jin-Xin He.Dye chemical[M].Beijing:China Textile Press,2005."
In Chinese" [2]Zhou Zhi,Summarization of Developing-out Theory and Colours of Dye-stuffs.College Journals of Nanchang.1996."
Materials Science Forum (Accepted)"In Chinese" [5] DU Peng, LI Luhai, ZHAO Wen, LENG Xian, HU Xuwei.
Advanced Materials Research (Accepted)"In Chinese" [6] Alexander Kamyshny, Matti Ben-Moshe, Shai Aviezer, Shlomo Magdassi .Ink-Jet Printing of Metallic Nanoparticles and Microemulsions[J].
Acknowledgement The study is financially supported by Key Project Funding of Beijing Municipal Education & Beijing natural science foundation Committee (KZ200910015001) and Funding Project for Academic Human Resources Development in Institutions of Higher learning under the jurisdiction of Beijing Municipality PHR(IHLB) (PXM2009-014223-077341);(PHR200909124); Opening investigation subject of Beijing area key lab of pringting & packaging material and technology (OISBKPP)(KF201010) References [1]Jin-Xin He.Dye chemical[M].Beijing:China Textile Press,2005."
In Chinese" [2]Zhou Zhi,Summarization of Developing-out Theory and Colours of Dye-stuffs.College Journals of Nanchang.1996."
Materials Science Forum (Accepted)"In Chinese" [5] DU Peng, LI Luhai, ZHAO Wen, LENG Xian, HU Xuwei.
Advanced Materials Research (Accepted)"In Chinese" [6] Alexander Kamyshny, Matti Ben-Moshe, Shai Aviezer, Shlomo Magdassi .Ink-Jet Printing of Metallic Nanoparticles and Microemulsions[J].
Online since: December 2011
Authors: Yun Tao Li, Jian Zhang, Zhi Hua Wang, Jun Wang, Yan Ping Zhao
Effect of Bell-type Annealing Process on Properties of SPCC Cold-rolled Steel Sheet
Zhihua WANG 1,2,a ,Yanping ZHAO 1,2,b ,Yuntao LI1,3 , Jun WANG4 ,
Jian ZHANG 1,2
1 School of Materials Science & Engineering, Tianjin University of Technology, Tianjin 300384, China
2 Key Laboratory of Display Materials & Photoelectric Devices (Tianjin University of Technology), Ministry of Education, Tianjin 300384, China
3 Tianjin Key Lab for Photoelectric Materials & Devices, Tianjin 300384, China
4 The State Key Lab for Rolling Technology and Automation of Northeast University, Shenyang 110004.
Experimental method Experimental material is SPCC cold-rolled sheet specimen with thickness of 0.5mm.
In addition, it also can be observed: As the annealing time increasing, the yield strength of this material decreased; but the change of the strain is more complex: when annealed for 3h, its strain value can reach the maximum, which is 47%, while the strain is relatively small for 1h and 5h.
Acknowledge: This work was supported by open found of the State Key Lab of Rolling Technology and Automation of Northeast University, and Tianjin Key Subject for Materials Physics and Chemistry.
Journal of University of Science and Technology Beijing., Vol27 (2005), p.532 [3] Guang Xu, Yi Yang, Xinqiang Zhang.
Experimental method Experimental material is SPCC cold-rolled sheet specimen with thickness of 0.5mm.
In addition, it also can be observed: As the annealing time increasing, the yield strength of this material decreased; but the change of the strain is more complex: when annealed for 3h, its strain value can reach the maximum, which is 47%, while the strain is relatively small for 1h and 5h.
Acknowledge: This work was supported by open found of the State Key Lab of Rolling Technology and Automation of Northeast University, and Tianjin Key Subject for Materials Physics and Chemistry.
Journal of University of Science and Technology Beijing., Vol27 (2005), p.532 [3] Guang Xu, Yi Yang, Xinqiang Zhang.
Online since: December 2014
Authors: Radim Lenort, David Holman, Pavel Wicher, David Staš
Journal of Production Economics. 118 (2009) 501-507
Journal of Production Economics. 140 (2012) 374-384
Djassemi, The Impact of Lean Level Material Flow on Performance of Manufacturing Systems for Large Complex Parts, Production and Inventory Management Journal 46 (2010) 127-35
Deuse, Levelling of low volume and high mix production based on a Group Technology approach, CIRP Journal of Manufacturing Science and Technology. 4 (2011) 247–251
Journal of Intell Manuf. 23 (2012) 49-60
Journal of Production Economics. 140 (2012) 374-384
Djassemi, The Impact of Lean Level Material Flow on Performance of Manufacturing Systems for Large Complex Parts, Production and Inventory Management Journal 46 (2010) 127-35
Deuse, Levelling of low volume and high mix production based on a Group Technology approach, CIRP Journal of Manufacturing Science and Technology. 4 (2011) 247–251
Journal of Intell Manuf. 23 (2012) 49-60