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Osteogenic Activity of MG63 Cells on Hydroxyapatite/Collagen Nanocompsosite under Pressure/perfusion Culture

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Abstract:

The purpose of this study is to analyze osteogenic activity of human osteoblastic MG63 cells on the bone-like self-organized hydroxyapatite/collagen (HAp/Col) nanocomposite sponge cultured by a pressure/perfusion technique using collagen sponge as a control. Histological analyses, alkaline phosphatase (AlkP) protein analysis and real-time polymerase chain reaction (PCR) analyses for AlkP and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) to evaluate the osteogenic activity of MG63. The MG63 cells were attached well and showed good proliferation on the HAp/Col sponge as well as in the control. The MG63 cells on HAp/Col sponge demonstrated higher osteogenic activity than those on the control. The results suggested that the HAp/Col sponge is expected to be a good scaffold for bone tissue engineering.

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Periodical:

Key Engineering Materials (Volumes 330-332)

Pages:

317-320

DOI:

https://doi.org/10.4028/www.scientific.net/KEM.330-332.317

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Online since:

February 2007

Authors:

Teruaki Yoshida, Masanori Kikuchi, Yoshihisa Koyama, Kazuo Takakuda

Keywords:

Hydroxyapatite/Collagen Nanocomposite Sponge, Osteogenic Activity, Pressure/Perfusion Culture

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© 2007 Trans Tech Publications Ltd. All Rights Reserved

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