Effect of DBP/PLGA Hybrid Scaffold on Angiogenesis during the Repair of Calvarial Bone Defect

Sun Jung Yoon; Ki Suk Park; Bang Sil Choi; Gil Son Khang; Moon Suk Kim; John M. Rhee; Hai Bang Lee

doi:10.4028/www.scientific.net/KEM.342-343.161

Paper Titles

Regeneration of Bone Defect Using Micro-Bioceramic PLLA Polymer Scaffolds Synthesized by Nonsolvent and Solvent Method
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Fabrication of Scaffold-Free Engineered Cartilage Using Passaged Chondrocytes in the Presence of Insuline Like Growth Factor 1 (IGF-1)
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Reinforcement Fibrin-Hyaluronic Acid Composite Gel for Tissue Engineering Cartilage Genesis
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RGD Direct Conjugation Using Co-Solvent System to Increase Cell Attachment on Poly(lactide-co-glycolide) without Change in Hydrophilic/Hydrophobic Property
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Effect of DBP/PLGA Hybrid Scaffold on Angiogenesis during the Repair of Calvarial Bone Defect
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In Vitro Proliferation of Various Human Dental Stem Cells
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Crosslinked Gelatin Nanofibers and their Potential for Tissue Engineering
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Hybrid Type of Tissue Engineered Biodisc Using Annulus Fibrosus Seeded PLGA Scaffold and Nucleus Pulposus Seeded MPEG-PCL Hydrogel: Preliminary Study
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Injectable Chitosan Carrier for Demineralized Bone Matrix
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Effect of DBP/PLGA Hybrid Scaffold on Angiogenesis during the Repair of Calvarial Bone Defect

Abstract:

This study was designed to investigate the influence of demineralized bone particles (DBP)/PLGA hybrid scaffold on angiogenesis and osteogenesis in a calvarial defect model. DBP/PLGA scaffolds were manufactured by solvent casting/salt leaching method, and each scaffold contained 0, 10, 20, 40, and 80 wt% DBP of PLGA, respectively. A total of 34 rats were operated and bicortical holes were placed on their calvaria. The defects were filled with different ratio DBP/PLGA scaffolds. After 3, 7, 14, and 28 days, specimens were taken and, histologic, immunohistologic and RT-PCR analyses were carried out concerning number of vessels and density of regenerated bone, and angiogenic activation. On days 7, in all experimental groups, bone formation occurred in a direction from defected margin of calvarium to center of implanted scaffold and new vessel formation took place in front of the osteogenic regeneration front. We found that the 20 and 40 wt% DBP/PLGA scaffold was superior in its ability to regenerate new bone, induced more intensive formation of microvasculature and expressed in a higher level of osteocalcin mRNA than other groups.