Paper Titles
Mechanical Properties of Living Cells: Interaction of the Adherent Endothelial Cell and the Fluid Shear Flow
p.1157
Preliminary Study for Evaluating Bone Forming Ability of Porous Bioceramics Using Rat Mesenchymal Stem Cells to be Used for International Standard
p.1161
Reconstruction of Liver Organoid Using an Apatite-Fiber Scaffold, a Radial-Flow Bioreactor and FCL-4 Cells of Hepatocyte Model
p.1165
Behavior of Cellularized-Hydroxyapatite Implants Coated with Cyclo-DfKRG Peptides in Spongious Bone: Quantitative Comparison of Micro-Tomodensitometry and Histomorphometry
p.1169
Expression of ODF and ICAM-1 of Bone Marrow Mesenchymal Stem Cells is Enhanced with Osteogenic Differentiation
p.1173
Stimulatory Effect of Nano-Sized Calcium Metaphosphate Particles on Proliferation and Osteoblastic Differentiation of Human Bone Marrow Mesenchymal Stem Cells
p.1177
In Vitro Studies of Cell Growth on Three Differently Fabricated Hydroxyapatite Ceramic Scaffolds for Bone Tissue Engineering
p.1181
In Vivo Osteogenesis in Porous Hydroxyapatite Scaffold Processed in Hyaluronic Acid Solution
p.1185
Osteogenic Influence of Lysine in Porous Hydroxyapatite Scaffold
p.1189

Expression of ODF and ICAM-1 of Bone Marrow Mesenchymal Stem Cells is Enhanced with Osteogenic Differentiation

Article Preview

Abstract:

Osteoblasts were perceived as pivotal cells, recognized as the cells that control both the formative and the resorptive phases of the bone remodeling cycle. Osteoblasts were an essential requirement for osteoclastogenesis though expressing or secreating bioactive osteoclast-differentiation-regulatory proteins, osteoclast differentiation factor (ODF)was the most important factor among these, ODF participate nearly in every step of differentiation and activation of osteoclasts. In addition, intercellular adhesion molecule-1 (ICAM-1)and its receptors LFA-1 play a role in osteoclast development by affecting adhesion between stromal cells and osteoclast progenitors before the occurrence of ODF-ODF receptor signaling. However, it is not clear about the relationship between ODF, ICAM-1 expression of osteoblasts and differentiation state of osteoblasts. So,the aim of this study was to investgate whether the expression of ODF, ICAM-1 depended on the stage of osteoblastic differentiation from rat bone marrow mesenchymal stem cells(rBMSCs). The viability of rBMSCs is reduced significantly by osteogenic inducement as differentiating into osteoblasts, ALPase activity of OS-treated rBMSCs was enhanced obviously within 9 days , declined subsequently and recovered nearly the original level at day 14. Expression of ODF is enhanced with osteogenic differentiation guadully. whereas, expression of ICAM-1 is activated at OS-treated day 6, then keeping at a stable level. This study indicated that rBMSCs undergoing osteogenic inducement was an ideal model for studying the differentiation and maturation of osteoblasts. During the early stage of differentiation along osteoblasts from stem cells to osteocytes, rBMSCs or Osteoprogenitor react somewhat differently from osteoblasts, suggesting the ability of osteoblasts to regulating differentiation and maturation of osteoclasts have been improved with osteogenic culture.

You might also be interested in these eBooks
Bioceramics 20 View Preview

Info:

Periodical:

Key Engineering Materials (Volumes 361-363)

Pages:

1173-1176

DOI:

https://doi.org/10.4028/www.scientific.net/KEM.361-363.1173

Citation:

Cite this paper

Online since:

November 2007

Authors:

Jun Wang, Yu Bo Fan, Zhi He Zhao, Juan Li, Jun Liu

Keywords:

Osteoblast, Osteogenic Differentiation, Stem Cell

Export:

RIS, BibTeX

Price:

Permissions CCC:

Request Permissions

Permissions PLS:

Request Permissions

Сopyright:

© 2008 Trans Tech Publications Ltd. All Rights Reserved

Share:

Citation:

References

[1] Aubin JE, Turkesen K, Herersche JN 1993 Osteoblastic cell lineage. In: Noda M (ed) Cellular and Molecular Biology of Bone. Academic Press, London, pp.1-45.

Google Scholar

[2] Lacey DL, Timms E, Tan HL, Kelley MJ. Cell 1998; 17; 93(2): 165-76.

Google Scholar

[3] Yasuda, H., Shima, N., Nakagawa, N., et al. Bone 1999. 25, 109-113.

Google Scholar

[4] Tanaka Y, Maruo A, Fujii K, et al. J Bone Miner Res 2000; 15(10): 1912-23.

Google Scholar

[5] Long P, Liu F, Piesco NP, et al. Bone 2002; 30(4): 547-52.

Google Scholar

[6] Kramer PR, Nares S, Kramer SF, et al. 2004; 83(1): 27-34.

Google Scholar

[7] Johnstone B, Hering TM, Caplan AI, et al. cells. Exp Cell Res 238: 265-272.

Google Scholar

[8] Mackay AM, Beck SC, Murphy JM, et al. Tissue Eng 1998; 4: 415-428.

Google Scholar

[9] Pittenger MF, Mackay AM, Beck SC, et al. Science 1999; 284: 143-147.

Google Scholar

[10] Maniatopoulos C, Sodek J, Melcher AH. Cell Tissue Res 1988; 254(2): 317-30.

Google Scholar

[11] Jaiswal N, Haynesworth SE, Caplan AI, et al. J Cell Biochem 1997; 64: 295-312.

Google Scholar

[12] Hofbauer LC, Khosla S, Dunstan CR, Lacey D, Boyle WJ, Riggs BL. J Bone Miner Res 2000; 15(1): 2-12.

Google Scholar

[13] TaniIshii N, Penninger JM, Matsumoto G, Teranaka T, Umemoto T. J Periodontal Res 2002; 37(3): 184-91.

Google Scholar