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Preparation of Porous Multi-Channeled Chitosan Conduits for Nerve Tissue Engineering

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Abstract:

A new method to fabricate porous chitosan nerve conduits with multi-channels was described. A uniquely designed mold was composed of 7-50 stainless steel needles and a set of plastic pedestals. Porous or imperforate chitosan tubes with 2-5mm inner diameter and 0.2-1.0 mm wall thickness were made firstly. The chitosan tubes were injected with 3% chitosan gel. The stainless steel needles longitudinally perforated through the chitosan tubes filled with chitosan gel, and the plastic pedestals were used to fix the needles. Lyophilization was used to finish fabrication. The diameter of channels was 0.2-0.4mm. Swelling property and biodegradability of Multi-channeled chitosan conduits were investigated. Wright’s staining and scanning electron microscope (SEM) were used to observe spread and proliferation of Neuroblastoma cells (N2A, mouse) on the conduits. It is promising that the porous chitosan nerve conduits with multi-channels are used as nerve tissue engineering scaffolds in repair of peripheral nerve and spinal cord injuries.

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Periodical:

Key Engineering Materials (Volumes 288-289)

Pages:

27-30

DOI:

https://doi.org/10.4028/www.scientific.net/KEM.288-289.27

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Online since:

June 2005

Authors:

Q. Ao, A.J. Wang, W.L. Cao, C. Zhao, Ya Dong Gong, N.M. Zhao, X.F. Zhang

Keywords:

Chitosan (CS), Lyophilization, Nerve Conduits, Tissue Engineering

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© 2005 Trans Tech Publications Ltd. All Rights Reserved

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References

[1] Den Dunnen WF, Meek MF, Grijpma DW, Robinson PH, Schakenraad JM: J Biomed Mater Res 2000; 51: 575 -585.

Google Scholar

[2] Kim SE, Park JH, Cho YW, Chung H, Jeong SY, Lee EB, Kwon IC: J Control Release 2003; 91(3): 365-374.

Google Scholar

[3] Teng YD, Lavik EB, Qu X, Park KI, Ourednik J, Zurakowski D, Langer R, Snyder EY: PNAS 2002; 99(5): 3024-3029.

Google Scholar

[4] Gong HP, Zhong YH, Li JC, Gong YD, Zhao NM, Zhang XF: J Biomed Mater Res 2000; 52: 285-295.

Google Scholar

[5] Li JC, Zhong YH, Gong HP, Yang M, Gong YD, Zhao NM, Zhang XF: The Fifth IUMRS International Conference on Advanced Materials, June 13-18, 1999, Beijing, China.

Google Scholar

[6] Madihally SV, Matthew HWT: Biomaterials 1999; 20: 1133-1142.

Google Scholar

[7] VandeVord PJ, Matthew HW, DeSilva SP, Mayton L, Wu B, Wooley PH: J Biomed Mater Res 2002; 59(3): 585-590.

DOI: 10.1002/jbm.1270

Google Scholar

[8] Zhang Y, Zhang MQ: J Biomed Mater Res, 2001; 55: 304-312.

Google Scholar

[9] Schoof H, Apel J, Heschel I, Gunter R: J Biomed Mater Res 2001; 58: 352-357.

Google Scholar

[10] Nam YS, Park TG: Biomaterials 1999; 20: 1783-1790.

Google Scholar