Papers by Keyword: Human Umbilical Vein Endothelial Cell

Abstract: Bio-electrospraying (BES) is becoming an attractive tool for the delivery of cells into scaffolds for tissue engineering applications. In this study, we aimed to electrospray human umbilical vein endothelial cells (HUVECs) and improve the efficiency of BES by designing a new customized multi-hole spinneret. We demonstrated that the multi-hole spinneret could produce continuous and stable jets during BES, and the efficiency was increased by 5–7 times. Morphological observations, trypan blue and sulforhodamine B assays revealed that the HUVECs electrosprayed using the multi-hole spinneret remained viable and proliferated at a rate similar to that of the controls. Thus, the new multi-hole nozzle can considerably improve output for BES without affecting cell morphology, viability, and proliferation.

Abstract: Natural antioxidants could inhibit ROS production and attenuate injury, which is important event of cardiovascular diseases. Water caltrop pericarps from three different Chinese water caltrop cultivars were collected and extracted using 70% methanol and hot water (WCPE). WCPE exhibited strong in vitro antioxidant activities tested by DPPH assay. Incubation with 100μM H2O2 for 1 h would reduce viability of HUVECs to 76.46±0.05%, decrease SOD, CAT and GSH-Px activities to 12.50±0.06, 10.08±0.57 and 107.98±2.68 U/mg protein compared with 20.57±0.02, 22.56±0.41 and 215.28±23.13 U/mg protein of normal cells, increase MDA content from 3.31±0.38 to 9.52±1.87 nmol/mg protein. Pre-treatment, post-treatment and simultaneous with WCPE could attenuate HUVECs injury and recover the viability to 107.77±0.06%, SOD, CAT and GSH-Px activities to 19.11±0.02, 26.14±0.89 and 192.62±7.75 U/mg protein and MDA content to 4.31±0.71 nmol/mg protein. These results suggested that WCPE attenuates H2O2-induced HUVECs injury through promoting proliferation, enhance antioxidant enzymes activities and reduce lipid peroxidation.

Abstract: In prior work we have shown that titanium oxide (Ti-O) thin films have good blood compatibility. However, as well as being hemocompatible, biomaterials used in contact with blood should be cell compatible also. In the work described here, Ti-O films were synthesized using unbalanced magnetron sputtering (UBMS) and were modified by immobilizing laminin on the film surface for improving human umbilical vein endothelial cell (HUVEC) adhesion and growth. Scanning electron microscopy (SEM), Fourier Transform Infrared spectroscopy (FTIR) and contact-angle measurements were used to investigate the surface characteristics of the Ti-O films and the modified Ti-O films. The results suggest that Laminin can be biochemically immobilized on the Ti-O film surface. The modified layer of Laminin can improve the hydrophilicity and wettability of Ti-O films. In vitro HUVEC investigations reveal that Laminin immobilized on the film surface greatly enhances cell adhesion and growth on Ti-O films.

Abstract: The objective of this work was to investigate cell adhesion, Poly Glycolic Acid (PGA) and PGA/ collagen nano-fibers on the silicone membrane. PGA with the weight-mixing ration of 40% was fabricated through the electronspun technique. The behaviors of Human Umbilical Vein Endothelial cells on these scaffolds are evaluated. The highest cell adhesion was observed in the PGA/collagen fibers with the diameter of 500 nm. This study indicates the effect of nano-fibers on the Human Umbilical Vein Endothelial cells for better understanding of interactions of cells with scaffold materials. Such information will have important implications for implantable vascular tissue engineering constructs.

Abstract: Poly (D,L-latic-co-glycolic acid) (PLGA) has been used as the artificial scaffold for blood vessel formation. In order to hinder smooth muscle cell (SMC) angiogenesis, new scaffold design method of loading Epigallocatechin 3-O-gallate (EGCG) on PLGA film was introduced. PLGA and EGCG were dissolved in acetone and film-shape scaffold was manufactured. Antiangiogenetic effect of EGCG released on scaffold was analyzed for SMC and human umbilical vein endothelial cell (HUVEC) and method for selective inhibition from the difference of growth of SMC and HUVEC was suggested.