Effects of Pulsed Electric Fields on Phospholipid Molecular Conformation by Raman Spectra

Abstract:

In order to study the sterilization mechanism of pulsed electric fields（PEF） on microorganisms and the interaction between PEF conditions and cell membrane composed of phospholipids, the authors studied the phospholipid molecular conformation changes under various electric fields strength treatments using Raman spectra. It was demonstrated from the Raman spectra that there was little influence of PEF on the conformation of O-C-C-N⁺ backbone in the choline group of phospholipid. The intensity ratios of I₁₀₉₆/I₁₀₆₂ and I₁₀₉₆/I₁₁₂₆ represented the gauche/ trans ratioof C-C vibrations, but the first was increased directly, while the other one was increased firstly and then decreased with increasing electric fields strengths but the ratio was still higher than that of the control sample. I₂₈₈₅/I₂₈₅₀ represeted order/ disorder of chain-chain interactions which was decreased with increasing electric fields strengths. It was indicated that the intermolecular disordering in hydrocarbon chain of the lipid lattice was increased and the membrane fluidity was enhanced after PEF treatments.

• Periodical: Applied Mechanics and Materials (Volume 140)
• Pages: 329-333
• DOI: https://doi.org/10.4028/www.scientific.net/AMM.140.329
• Online since: November 2011
• Authors: Xiao Jia, Xin An Zeng
• Keywords: Conformation, Phospholipid Molecular, Pulsed Electric Field (PEF), Raman Spectra, Sterilization Mechanism

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