Effect of L-cysteine on Continuous Fermentative Hydrogen Production

Abstract:

Two continuously stirred tank reactors (CSTR) were used to investigate the effect of L-cysteine on continuous dark fermentative hydrogen production system. L-cysteine was added into one of the reactors at 0.1 g/L continuously, the other reactor was without L-cysteine addition. Liquid end products, oxidation-reduction potential (ORP), pH, biomass and hydrogen production rate (HPR) were examined during the operation. The lag time of ethanol generation in the L-cysteine added reactor was shortened to 21 days compared to 25 days in the blank. The HPR increased from 2.73 L/d in the reactor without L-cysteine addition to 2.82 L/d in the added one. The volatile suspended solids (VSS) in the reactor with L-cyateine was 10.1 g/L by the end of the operation compared to 9.2 g/L in the other. Addition of L-cysteine into the hydrogen production system accelerates the formation of ethanol-type fermentation and enhances the hydrogen production by creating an optimal low ORP environment, and by increasing the biomass growth simultaneously.

• Periodical: Applied Mechanics and Materials (Volumes 178-181)
• Pages: 406-410
• DOI: https://doi.org/10.4028/www.scientific.net/AMM.178-181.406
• Online since: May 2012
• Authors: Yuan Yuan Qu, Wan Qian Guo, Jie Ding, Nan Qi Ren
• Keywords: Anaerobic Fermentation, Biohydrogen, Continuous Hydrogen Production, L-Cysteine

References

[1] F. R. Hawkes, I. Hussy, G. Kyazze, R. Dinsdale, and D. L. Hawkes: Int. J. Hydrogen Energy. Vol. 32 (2007), p.172

DOI: 10.1016/j.ijhydene.2006.08.014

[2] H. Zhu, W. Parker, R. Basnar, A. Proraki, P. Falleta, M. Beland, and P. Seto: Int. J. Hydrogen Energy. Vol. 33 (2008), p.3651

[3] N. Q. Ren, H. Chua, S. Y. Chan, Y. F. Tsang, Y. J. Wang, and N. Sin: Bioresour. Technol. Vol. 98 (2007), p.1774

[4] M. H. Hwang, N. J. Jang, S. H. Hyun, and I. S. Kim: J. Biotechnol. Vol. 111 (2004), p.297

[5] C. Y. Lin, C. C. Wu, J. H. Wu and F. Y. Chang: Biomass and Bioenergy. Vol. 32 (2008), p.1109

[6] Y. Song and B. E. Logan: Water Res. Vol. 38 (2004), p.1626

[7] M. T. Skonieczny and V. Yargeau: Int. J. Hydrogen Energy. Vol. 34 (2009), p.3288

[8] H. Q. Chen, X. X. Ma, D. D. Fan, Y. E. Luo, P. F. Gao and C. Y. Yang: Chinese Journal of Chemical Engineering. Vol. 18 (2010), p.681

[9] Z. L. Yuan, H. J. Yang, X. H. Zhi and J. Q. Shen: Int. J. Hydrogen Energy. Vol. 33 (2008), p.6535

[10] American Public Health Association (APHP). Standards methods for the examination of water and wastewater. 20th ed. Washington DC, America (1998)

[11] J. H. Hwang, J. A. Choi, R. A. I. Abou-Shanab, A. Bhatnagar, B. Min, H. Song, E. Kumar, J. Choi, E. S. Lee, Y. J. Kim, S. Um, D. S. Lee, and B. H. Jeon: Int. J. Hydrogen Energy. Vol. 34 (2009), p.9702

DOI: 10.1016/j.ijhydene.2009.10.022

[12] N. Q. Ren, B. Z. Wang, and F. Ma, in: Proceedings of the 68th water environment federation annual conference exposure (1995), p.145

[13] J. Z. Li, B. K. Li, G. F. Zhu, N. Q. Ren, L. X. Bo and J. G. He: Int. J. Hydrogen Energy. Vol. 32 (2007), p.3274

[14] X. M. Liu, N. Q. Ren, F. N. Song, C. P. Yang and A. J. Wang: Progress in Natural Science. Vol. 18 (2008), p.253