Effects of Additional Stimulants on Phenol Degradation by a Halotolerant Rhodococcus sp. JDD1H

Abstract:

A halotolerant and highly effective phenol-degrading strain, named as JDD1H, was isolated from soil contaminated by petroleum and identified as Rhodococcus sp. Phenol degradation by strain JDD1H in the presence of high salinity, especially the effect of exotic stimulants such as carbon source, nitrogen source and surfactant on phenol degradation was investigated. The results show that strain JDD1H could utilize phenol as sole carbon and energy source to grow in the presence of 5% NaCl with 16 h adaptive phase. The degradation efficiency of 150 mg/L phenol was up to 99.5% within 40 h. Lactose, cane sugar, glucose and mannitol in wide range of 100~2000 mg/L enhanced phenol degradation significantly. Organic nitrogen sources, as peptone and yeast extract, and inorganic nitrogen sources, as NH4Cl and (NH4)2SO4, accelerated phenol degradation. Tween-80 with concentration of 50-1000 mg/L restrained phenol degradation and Tween-80 of 1500-2000 mg/L could promote degradation.

• Periodical: Applied Mechanics and Materials (Volumes 253-255)
• Pages: 914-920
• DOI: https://doi.org/10.4028/www.scientific.net/AMM.253-255.914
• Online since: December 2012
• Authors: Fang Yan Chen, Duo Duo Jiang, Yu Bin Tang
• Keywords: Biodegradation, Carbon Source, Nitrogen Source, Phenol, Rhodococcus Sp., Surfactant

References

[1] N. Massalha, A. Shaviv and I. Sabbah: Journal of Water Research, Vol. 44 (2010), p.5252

[2] K. S. Zhang, M. Shi, Z. Zhang and T. Fang: International Journal of Environment and Pollution, Vol. 32 (2008), p.68

[3] H. J. Liu, X. P. He and M. Zhang: Journal of Genomics and Applied Biology, Vol. 30 (2011), p.336(in Chinese)

[4] Information on http://www. epa.gov/waterscience/methods/pollutants.htm.last

[5] V. Arutchelvan, V. Kanakasabai and S. Nagarajan: Journal of Hazardous Materials, Vol. B127 (2005), p.238

[6] A. Kumar, S. Kumar and S. Kumar: Biochem. Eng. J., Vol. 22 (2004), p.151

[7] L. Leven and A. Schnurer: International Biodeterioation &Biodegradation, Vol. 64 (2010), p.427

[8] R. S. Juang and C. Y. Wu: Chemosphere, Vol. 66 (2007), p.191

[9] S. I. Abou-Elela, M. M. Kamel and M. E. Fawzy: Desalination, Vol. 250 (2010), p.1

[10] C. Gao, Q. S. Wu and H. Y. Xia: Industrial Water Treatment, Vol. 33 (2011), p.9(in Chinese)

[11] M. F. Han and. J. L. Yang: Hebei Chemical Engineering, Vol. 30 (2007), p.59(in Chinese)

[12] R. B. Cardoso, A.C. Texier, Á.A. Solís, J. Gómez and E.R. Flores: Process Biochemistry, Vol. 44 (2009), p.223

[13] J. H. Jeon, H. S. Lee, J.T. Kim and S. J. Kim: Journal of Applied Microbiology and Biotechnology, Vol. 93 (2012), p.623

[14] L.F. Qiu, F. F. Wu and Y. Yao: Shanghai Environmental Sciences, Vol. 2 (2010), p.35 (in Chinese)

[15] X. Y. Guan: Ph D Thesis, Dalian University of Technology, (2007) (in Chinese)

[16] A. Banerjee and A. K. Ghosha: Journal of Hazardous Materials, Vol. 176 (2010), p.85

[17] Z. Xiao, F. Huo and Y. Huang: Bioresource Technology, Vol. 114 (2012), p.59

[18] Z. J. Zhai and S. B.Yan: Journal of Chemical Technology and Biotechnology, Vol. 87 (2012), p.105

[19] H. Y. Wen: Ph D Thesis, China University of Mining and Technology, (2009) (in Chinese)

[20] Y. Y. Qu, H. Zhou and P. Wang: Journal of Dalian University of Technology, Vol. 51 (2011), p.25 (in Chinese)

[21] L.Yue, Z.Tang and Y. Song: Journal of Journal of China West Normal University (Natural Science) Vol.33 (2012), p.146 (in Chinese)

[22] J. Wang and L. C. Jiang: Jiangsu Agriculture Science, Vol.4 (2012), p.311 (in Chinese)

[23] F. X.Yang and Y. Tang: Journal of China West Normal University (Natural Science), Vol.29 (2008), p.144 (in Chinese)

[24] Y. Ding, X. Z.Yuan and G. M. Zeng: Environmental Science, Vol.31 (2010), p.1047(in Chinese)

[25] L. Zhao, J. Y. Zhang and Z. W. Xu: Environmental Science and Technology, Vol.29 (2006), p.4 (in Chinese)