Comparison of Extraction Methods for Quantifying Extracellular Polymers of Marine Algae

Li Yun Ge; Yong Gang Huang; De Xiang Gao; Huan Huan Deng

doi:10.4028/www.scientific.net/AMM.260-261.1173

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HomeApplied Mechanics and MaterialsApplied Mechanics and Materials Vols. 260-261Comparison of Extraction Methods for Quantifying...

Comparison of Extraction Methods for Quantifying Extracellular Polymers of Marine Algae

Abstract:

Five extraction methods were used to extract extracellular polymers (EPS) from Platymonas subcordiformis. Results suggested that the extraction efficiencies of EPS decreased as followed: cation exchange resin (CER) > ultrasonic extraction> heating (80°C) > EDTA > centrifugation. CER method was the most effective in the EPS extraction from P. subcordiformis with the highest amount of EPS and the lowest cell lysis, while the ultrasonic extraction method was not suitable to extract EPS for its obvious cell lysis. In addition, heating (80 °C) was used to investigate EPS formation of P. subcordiformis. Results showed EPS amount increased at the increased incubation time when the nutrients were enough whereas the ratio of EPS components remained unchanged in the growing cycle.

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Periodical:

Applied Mechanics and Materials (Volumes 260-261)

Pages:

1173-1178

DOI:

https://doi.org/10.4028/www.scientific.net/AMM.260-261.1173

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Online since:

December 2012

Authors:

Li Yun Ge, Yong Gang Huang, De Xiang Gao, Huan Huan Deng

Keywords:

Carbohydrate, Extracellular Polymers, Marine Algae, Platymonas Subcordiformis, Protein

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References

[1] S. Tsuneda, S. Park and H. Hayashi: Water Sci Technol Vol. 43 (2001), p.197.

Google Scholar

[2] J. F. Gao, Q. A. Zhang, J. H. Wang, X. L. Wu, S. Y. Wang and Y. Z. Peng: Bioresour. Technol Vol. 102 (2011), p.805.

Google Scholar

[3] B. Frolund, T. Griebe and P. H. Nielsen: Appl Microbiol Biotechnol Vol. 43 (1995), p.755.

Google Scholar

[4] P. Cescutti, R. Toffanin, P. Pollesello and I. W. Sutherland: Carbohydrate Research Vol. 315 (1999), p.159.

Google Scholar

[5] R. Bura, M. Cheung, B. Liao, C. Finlayson and B. C. Lee: Water Sci Technol Vol. 37 (1998), p.325.

Google Scholar

[6] J. Wingender, T. R. Neu and H. C. Flemming, Microbial extracellular polymeric substances: characteri- zation, structures and function. Springer-Verlag, Berlin Heidelberg, Chapter 3, (1999).

DOI: 10.1007/978-3-642-60147-7

Google Scholar

[7] D. J. Smith and G. J. C. Underwood: Limnol Oceanogr Vol. 43 (1998), p.1578.

Google Scholar

[8] K. D. Hoagland, J. R. Rosowski, M. R. Gretz and S. C. Roemer: J Phycol Vol. 29 (1993), p.537.

Google Scholar

[9] L. Y. Ge, H. H. Deng, H. W. Gao and F. Wu: Res. J Chem Environ Vol. 14 (2010), p.78.

Google Scholar

[10] M. Mejáre and L. Bülow: Trends Biotechnol. Vol. 19 (2001), p.67.

Google Scholar