The principle of remote parameter control(RPC) and test method of road simulator test are analyzed, and the road simulation test of motorcycle is implemented with road simulator produced by MTS company. Firstly, by disposing strain gauge and accelerometer, the load spectrum of motorcycle is sampled when the motorcycle runs on gravel road and concrete road with constant speed. Then using the front and rear axle acceleration as desired response signals, the simulation iteration and road simulation test are conducted. At last, the key problems of motorcycle road simulation test are solved. The result shows that the desired response signals and the monitor signals are well matched, and the road load spectrum of motorcyle is accurately reproduced.