Treatment of Urea Waste Water over Coconut Shell Activated Carbon Tethered Urease

Abstract:

Urease was successfully tethered onto coconut shell activated carbon (AC) granules. Degradation of urea was carried out in packed bed over the tethered urease. For 75% of retained activity, the tethered urease shows a broader temperature range of 42~80°C, compared to 45~75°C for the free enzyme. Similarly, the tethered urease has an increased resistance against the changes of pH. For a relative activity of 80%, the free urease had a pH range of 6.2-7.4, while it was 6.5–8.0 for the tethered urease. The Km values of the free and tethered ureases were 22.60 mg/mL and 7.43 mg/mL, respectively. And the Vm values were actually very close (~23.5 mg/min ) for both the tethered and free ureases. The catalytic performance of the tethered urease was tested in a packed bed reactor. The relative activity was maintained over 80% after 50 h of running in degradation of urea.

• Periodical: Applied Mechanics and Materials (Volumes 295-298)
• Pages: 1359-1363
• DOI: https://doi.org/10.4028/www.scientific.net/AMM.295-298.1359
• Online since: February 2013
• Authors: Lei Wang, Sha Wang, Di Xiong, Chun Rong Xiong
• Keywords: Coconut Shell Activated Carbon (AC), Immobilisation, Packed Bed Reactor, Urease

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