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Improvement of Daptomycin Yield by Overexpression of ATP-Binding Cassette (ABC) Transport Proteins Genes of Daptomycin Gene Cluster

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Abstract:

The effects of the genes of ORF53-55, which code ATP-binding cassette (ABC) transport proteins, on daptomycin production were investigated by overexpression. The yield of daptomycin was promoted significantly when either of the genes of ORF53, ORF54 or ORF55 was overexpressed individually or by a combined manner. The results above suggested that the ORF53, ORF54 and ORF55 genes had positive cooperativity in the biosynthesis of daptomycin. The dry weight and sugar utilization of HP-ORF53-55 is significantly improved as compared with the LC-54-16. Based on these results, it was speculated that the genes of ORF 53, 54 and 55 may play an important positive role in the process of reducing sugar transport, which enhanced the cell growth and daptomycin biosynthesis.

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Periodical:

Applied Mechanics and Materials (Volumes 295-298)

Pages:

164-168

DOI:

https://doi.org/10.4028/www.scientific.net/AMM.295-298.164

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Online since:

February 2013

Authors:

Guang Hai Yu, Yu Tao Min, Yuan Sen Hu, Yan Li Yin, Liang Huang

Keywords:

ABC Transport Genes, Daptomycin Production, Overexpression, S. Roseosporus

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© 2013 Trans Tech Publications Ltd. All Rights Reserved

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