Plasma Membrane Stability Analysis of Cold-Area Winter Wheat

Abstract:

Four varieties of winter wheat with different return green rates were used to analyze the plasma membrane stability and the factors that affect cold-area winter wheat during winter. The removal of reactive oxygen species, the degree of plasma membrane impairment, water content in the crowns, which contain the growing point, were studied during cold acclimation to the deep freezing period. The results show that electrical conductivity reflects the degree of plasma membrane damage under low temperatures was significantly correlated with the free water and the total water content. The MDA content, which reflects the degree of membrane peroxidation, was very significantly correlated with SOD, peroxidase, and ascorbic acid. During this period, the MDA stability, SOD, GSH, and total water and free water content may be used to identify the cold resistance of winter wheat varieties.

• Periodical: Applied Mechanics and Materials (Volumes 316-317)
• Pages: 1094-1100
• DOI: https://doi.org/10.4028/www.scientific.net/AMM.316-317.1094
• Online since: April 2013
• Authors: Xiao Nan Wang, Dong Wei Xie, Lian Shuang Fu, Zhuo Fu Li, Ying Lu Sun
• Keywords: Cold Resistance, Plasma Membrane Peroxidation, Protective Enzyme, Winter Wheat

References

[1] Wang X N, Fu L S, Li Z F, Sun YL, Liu C, Wang J W, Chen Y X.2009.Morphogenesis and physiological basis in wheat cultivars with different levels of cold-resistance during cold acclimation and freezing period.Acta Agronomica Sinica ,35,1313-1319(in Chinese).

DOI: 10.3724/sp.j.1006.2009.01313

[2] Yu J，Zhang L, Cui H, Zhang Y X, Cang J，Hao Z B.2008.Physiological and biochemical characteristics of Dongnongdongmai 1 before wintering in high-cold area.Acta Agronomica Sinica ,34,2019-2025(in Chinese).

DOI: 10.3724/sp.j.1006.2008.02019

[3] Geert P, Nele H, Marcel A K J.2010. The cellular redox state in plant stress biology-A charging concept.Plant Physiology and Biochemistry ,48,292-300.

DOI: 10.1016/j.plaphy.2009.12.007

[4] Yamamoto Y，Kobayashi Y，Matsumoto H .2001.Lipid peroxidation is an early symptom triggered by aluminum,but not the primary cause of elongation inhibition in pea roots.Plant Physiol ,125,199-208.

DOI: 10.1104/pp.125.1.199

[5] Jian LC ,Wan H.2009. Stress plant cell biology.Science Press, Beijing，pp,133(in Chinese).

[6] Zou Q.2000. Plant physiological biochemical experimental guidance.China Agriculture Press, Beijing，pp,4-5(in Chinese).

[7] Li L.2009. Plant physiology module experimental guidance. Science Press, Beijing, pp,76-77 .

[8] Hodges D M，Delong J M.1999.Improving the thiobarbituric acid-reactive-substances assay for estimating lipid peroxidation in plant tissues containing anthocyanin and other interfering compounds .Planta ,207,604-611.

DOI: 10.1007/s004250050524

[9] Giannopolitis C N, Ries SK.1977.Superoxide dismutases: I. Occurrence in higher plants.Plant Physiology, 59,309-314.

DOI: 10.1104/pp.59.2.309

[10] Cakmak I, Marschner H.1992.Magnesium deficiency and high light intensity enhance activities of superoxide dismutase, ascorbate peroxidase, and glutathione reductase in bean leaves. Plant Physiology ,98,1222-1227.

DOI: 10.1104/pp.98.4.1222

[11] Nagalakshmi N, Prasad M N V.2001.Responses of glutathione cycle enzymes and glutathione metabolism to copper stress in Scenedesmus bijugatus．Plant Science ,160,291-299.

DOI: 10.1016/s0168-9452(00)00392-7

[12] Tanaka K, Suda Y, Kondo N.1985.Ozone tolerance and the ascorbate-dependent hydrogen peroxide decomposing system in chloroplasts.Plant Cell Physiol ,26,1425-1431.

[13] Wan FS.2006.Winter wheat varieties trans-winter modified theory and practice.China Agriculture Press，Beijing，pp,43-45(in Chinese).

[14] Abdul J C, Sankar B, Murali P V, Gomathinayagam M, Lakshmanan GMA, Panneerselvam R.2007.Water deficit stress effects on reactive oxygen metabolism in Catharanthus roseus: impacts on ajmalicine accumulation. Colloids and Surfaces B: Biointerfaces ,62,105-111.

DOI: 10.1016/j.colsurfb.2007.09.026

[15] Xu F J, Jin C W, Liu W J, Zhang Y S, Lin X Y.2011. Pretreatment with H2O2 alleviates aluminum-induced oxidative stress in wheat seedlings.Journal of Integrative Plant Biology, 53,44–53.

DOI: 10.1111/j.1744-7909.2010.01008.x

[16] Bowler C, Van M M, Inze D.1992.Superoxide dismutase and stress tolerance.Annu Rev Plant Physiol PlantMol Biol ,43,83-116.

DOI: 10.1146/annurev.pp.43.060192.000503

[17] Guo Z, Ou W，Lu S, Zhong Q.2006.Differential responses of antioxidative system to chilling and drought in four rice cultivars differing in sensitivity. Plant Physiol Biochem ,44,828-836

DOI: 10.1016/j.plaphy.2006.10.024

[18] Ian M M. 2001. Plant mitochondria and oxidative stress electron transport NADPH turnover and metabolism of reactive oxygen species.Annu.Rev.Plant Physiol Plant Mol.Biol ,52,561-591.

DOI: 10.1146/annurev.arplant.52.1.561

[19] Xiang K, Zhang M Y, Xu Y, Wang X F, Yue LX.2011.Cold-tolerance of walnut cultivars: A comprehensive evaluation. Chinese Journal of Applied Ecology ,22,2325-2330(in Chinese).

[20] Zhou B R, Zhang Y F, Zhang MX, Chen T, Li Z.2008. Seasonal changes in leaf metabolites of two Sabina species and their relations with frost tolerance. Journal of Glaciology and Geocryology ,30,351-355(in Chinese).