Isolation and Identification of High Efficient Oil-Degrading Bacteria and Formulation of Complex Bacteria

Abstract:

In this experiment, 25 oil degrading bacteria strains were isolated from the beach near Dalian Bay. The top 4 high efficient oil-degrading bacteria（SQ、SY 、SW、SC） were selected to be studied. Using Physiological and biochemical identification, electron microscopy, and 16S rDNA sequence analysis, strain SQ was identified as Advenella kashmirensis，SY was identified as Achromobacter xylosoxidans, SW was identified as Acinetobacter venetianus，SC was identified as Alcaligenes xylosoxidans subsp.xylosoxidans. For the first time this experiment provided reference to application and degradation characteristics of Advenella kashmirensis in crude oil treatment. These four marine oil degrading bacteria were selected to form bacterial consortium. The most efficient mixed strains were constructed by using these four strains with proportional combination. The oil removal ratio of the optimal mixed strains increased to 70.33%, nearly 25% higher than the highest degradation rate of single bacterium, showing significant synergism in the bacterial consortium. Advenella kashmirensis enhanced oil degradation obviously, playing a crucial role in the bacterial consortium.

• Periodical: Applied Mechanics and Materials (Volumes 316-317)
• Pages: 594-598
• DOI: https://doi.org/10.4028/www.scientific.net/AMM.316-317.594
• Online since: April 2013
• Authors: Wen Jing Hao, Jun Mu, Yuan Li
• Keywords: Advenella kashmirensis, Bacterial Consortium, Marine Oil-Degrading Bacteria

References

[1] Mmilili M. Mapolelo, Ryan P. Rodgers ,Greg T. Blakneyb, Andrew T. Yenc, Sam Asomaningc, Alan G. Marshall ,2011. Characterization of naphthenic acids in crude oils and naphthenates by electrospray ionization FT-ICR mass spectrometry. International Journal of Mass Spectrometry, 300: 149--157.

DOI: 10.1016/j.ijms.2010.06.005

[2] Kate A. Harris, Mark B. Yunker, Neil Dangerfield, Peter S. Ross, 2011.Sediment-associated aliphatic and aromatic hydrocarbons in coastal British Columbia, Canada: Concentrations, composition, and associated risks to protected sea otters. Environmental Pollution, 159:2665--2674.

DOI: 10.1016/j.envpol.2011.05.033

[3] Mu-tai Bao , Li-na Wang, Pei-yan Sun , Li-xin Cao b, Jie Zou , Yi-ming Li, 2012. Biodegradation of crude oil using an efficient microbial consortium in a simulated marine environment. Marine Pollution Bulletin, 64(6):1177--1185.

DOI: 10.1016/j.marpolbul.2012.03.020

[4] Wang Weiping, Li Wei, Qiu Yanlin, Zhang Huashan, Peng Xiaobin, 2009. Isolation and identification of high efficient petroleum degrading bacteria .Chemistry & Bioengineering,26(7).

[5] Xi Danli, Sun Yusheng, Liu Xiuying, 1995. environmental monitoring. Higher Education Press,583--585.

[6] Olga Sánchez, Elia Diestra, Isabel Esteve, Jordi Mas, 2005. Molecular Characterization of an Oil-Degrading Cyanobacterial Consortium. Microbial Ecology , 50: 580--588.

DOI: 10.1007/s00248-005-5061-4

[7] Seonghun Kim, Sun Bok Lee, 2008. Identification and Characterization of the Bacterial D-Gluconate Dehydratase in Achromobacter xylosoxidans .Biotechnology and Bioprocess Engineering, 13: 436--444.

DOI: 10.1007/s12257-008-0152-y

[8] Wriddhiman Ghosh, Angshuman Bagchi, Sukhendu Mandal, Bomba Dam and Pradosh Roy ,2005. Tetrathiobacter kashmirensis gen. nov.,sp. nov., a novel mesophilic, neutrophilic, tetrathionate-oxidizing, facultatively chemolithotrophic betaproteobacterium isolated from soil from a temperate orchard in Jammu and Kashmir, India. International Journal of Systematic and Evolutionary Microbiology, 55: 1779--1787.

DOI: 10.1099/ijs.0.63595-0

[9] William J. Hunter, Daniel K. Manter, 2008. Bio-Reduction of Selenite to Elemental Red Selenium by Tetrathiobacter kashmirensis. Current Microbiology, 57(1):83--88.

DOI: 10.1007/s00284-008-9160-6

[10] Federici E, Pepi M, Esposito A, Scargetta S, Fidati L, Gasperini S, Cenci G, Altieri R, 2011. Two-phase olive mill waste composting: community dynamics and functional role of the resident microbiota. Bioresour Technol. 102(23):109 65--72.

DOI: 10.1016/j.biortech.2011.09.062

[11] Yang Jin-feng, Cao Guan-nan , Yang Zheng-li, 2011 .Isolation and Identification of Diesel Degradation Strain in Sandbox .Journal of GuiLin University of Technology, 31(3).