Spectrophotometric Determination of Trace Hg²⁺ with hsDNA-Modified Nanosilver Probe

Abstract:

Herring sperm DNA (hsDNA) was used to modify 5 nm nanosilver to obtain a hsDNA-nanosilver probe (Ag-hsDNA) for Hg²⁺. In the presence of Hg²⁺, it combined with hsDNA to produce Hg²⁺-hsDNA complexes and to release nanosilver particles, based on formation of stable T-Hg²⁺-T mismatches. Nanosilver particles aggregated to form larger nanosilver clusters and led the absorption at 412 nm decreased in the pH 6.5 Na₂HPO^4-NaH₂PO₄ buffer solution and 0.05 mol/L NaCl medium. The decreased absorption (ΔA₄₁₂ nm) is linear to Hg²⁺ concentration in the range of 1.36-10.86μg/L Hg²⁺, with regress equation of ΔA=0.0987CHg²⁺ +0.0624, correlation coefficient of 0.9890, and detection limit of 0.30μg/L Hg²⁺. The assay was applied to the analysis of Hg²⁺ in wastewater with satisfactory results.

• Periodical: Applied Mechanics and Materials (Volume 319)
• Pages: 513-516
• DOI: https://doi.org/10.4028/www.scientific.net/AMM.319.513
• Online since: May 2013
• Authors: Xiao Jing Liang, Gui Qing Wen, Zhi Liang Jiang
• Keywords: Hg²⁺, hsDNA-Modified, Nanosilver Probe, Spectrophotometry

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