Properties of Fiber Optic DNA Sensor Based on Fluorescence Quenching Using Lock-in Amplifier

Abstract:

To detect DNA concentration, a novel fiber optic biosensor based on fluorescence quenching was designed and constructed by using lock-in amplifier for signal measurement. A linear relationship between the relative phase delay Δφ of the sensing membrane and calf thymus DNA (ctDNA) concentration was observed in range from 2.0×10^-9 to 4.0×10^-7 M in the buffer of 5.0 mM Tris-HCl and 50.0 mM NaCl (pH 7.1), and the detection limit was 8.0×10^-10 M. The biosensor has a response time of 65 s and shows good stability and repeatability.

• Periodical: Applied Mechanics and Materials (Volumes 373-375)
• Pages: 302-305
• DOI: https://doi.org/10.4028/www.scientific.net/AMM.373-375.302
• Online since: August 2013
• Authors: Hua Fang, You Rong Wang, Li Yun Ding
• Keywords: ctDNA, Fluorescence Quenching, Property, Sensor

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