Picomol Assay of Nitric Oxide Screening in Biological Samples by Derivatization Combined with High-Throughput Microplate Format

Abstract:

Nitric oxide (NO) is a critical intra-and extra-cellular signaling molecule that mediates a broad variety of physiologic and pathophysiologic events, and has prompted rapid growth of investigations in investigating its physiology and generation mechanism of corresponding diseases. In this paper, a picomol assay of NO screening method using fluorescent derivatization combined with high-throughout microplate format has been developed and successfully applied to the determination of NO in biological samples. The satisfied detection limit and recovery of the proposed method demonstrates that it can be competent for the sensitive determination of NO in extremely small volume of biological sample.

• Periodical: Applied Mechanics and Materials (Volumes 423-426)
• Pages: 1786-1789
• DOI: https://doi.org/10.4028/www.scientific.net/AMM.423-426.1786
• Online since: September 2013
• Authors: Xiao Ling Zhang, Qiao Yang
• Keywords: Biological Samples, Derivatization, High-Throughput, Microplate Format, Nitric Oxide

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