Identification and Monitoring Strategy of Macrophomina phaseolina from Imported Soybeans

Abstract:

The isolate MP-63280 was isolated from imported soybean in Shanghai port by traditional PDA method. The isolate grew fast, produced typical black microsclerotia. Universal primers and special primers were used to amplify and sequence from MP-63280. Compared with the identity of rDNA sequence of Macrophomina phaseolina from NCBI GenBank databases by blast method, the result showed that its homology was up to 99%. Pathogenicity tests showed that soybean, cotton and sweet potato were infected by MP-63280. Based on morphological characteristics, molecular and pathogenicity test results, the isolate was identified as Macrophomina phaseolina (Tassi) goid. The effective methods to prevent the fungi from spread and transimission are to strengthen inspection and quarantine.

• Periodical: Applied Mechanics and Materials (Volumes 423-426)
• Pages: 353-357
• DOI: https://doi.org/10.4028/www.scientific.net/AMM.423-426.353
• Online since: September 2013
• Authors: Juan Sun, Shun Sheng Chen, Cui Yu, Cui Yun Yang
• Keywords: Identification, Imported Soybean, Macrophomina phaseolina, Monitoring Strategy

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