Culturing and Bonding of Diatom on a Microfluidic Chip for Biosensing Application

Abstract:

In order to fabricate biosensors with high sensitivity, the diatom Coscinodiscus excentricus was cultured on a microfluidic chip made with polydimethylsiloxane (PDMS), then the organic matter of diatom cells was removed and only diatom frustules were left, after which the cleaned frustules were bonded onto the bottom of the PDMS micro chamber by ultraviolet irradiation. The overview and the detailed morphology of the frustules in the chamber were analyzed by SEM, and the ultraviolet irradiation bonding mechanism of diatom with PDMS was discussed. Finally, the diatom based microfluidic chip was tested with the fluorescein-labeled protein; and the result showed that the fluorescence intensity of the diatom is 4 times stronger compared with that of the blank regions.

• Periodical: Applied Mechanics and Materials (Volume 461)
• Pages: 809-813
• DOI: https://doi.org/10.4028/www.scientific.net/AMM.461.809
• Online since: November 2013
• Authors: Jun Cai, Jun Feng Pan, Ming Li Chen, Yu Wang, De Yuan Zhang
• Keywords: Biosensing, Biosensor, Diatom, Hierarchical Structure, Microfluidic Chip

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