Aerobic Production of Butanol with Bacillus amyloliquefaciens NELB-12

Dina El-Hadi; Zong Ming Zheng; Chang Qing Dong

doi:10.4028/www.scientific.net/AMM.473.105

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HomeApplied Mechanics and MaterialsApplied Mechanics and Materials Vol. 473Aerobic Production of Butanol with Bacillus...

Aerobic Production of Butanol with Bacillus amyloliquefaciens NELB-12

Abstract:

n-butanol is a basic chemical compound with lower volatility, intersolubility and higher heating value, making it suitable to be used as a potential alternative biofuel. One butanol producing strain was isolated from soil and identified by 16S rDNA sequencing. Two universal primers (27F, 1492R) were used. Squence analysis indicated 16S rDNA sequence (Accession Number KF418240) of this strain was 99% identical to that of Bacillus amyloliquefaciens. This strain was designed as Bacillus amyloliquefaciens NELB-12. Optimaization of fermentation medium composition and fermentation conditions were carried out. The optimal medium main components were 30 g/l starch, 4 g/l ammonium nitrate, and 30 g/l beef extract. The optimal fermentation cultured with working volume of 120 ml in 250 ml flask at pH 6.5, 39°C, and 100 rpm. Bacillus NELB-12 could produce butanol at higher concentration that reached 8.9 g/l with a total ABE of 12.7 g/l and showed a high butanol tolerance. B. amyloliquefaciens NELB-12 is considered as an economical and cost effective potential producer for butanol industry.

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Periodical:

Applied Mechanics and Materials (Volume 473)

Pages:

105-110

DOI:

https://doi.org/10.4028/www.scientific.net/AMM.473.105

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Online since:

December 2013

Authors:

Dina El-Hadi, Zong Ming Zheng, Chang Qing Dong

Keywords:

16SrDNA, Aerobic, Bacillus amyloliquefaciens, Butanol, Fermentation, Gas Chromatography

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References

[1] Teck Khiang Chua, Da-Wei Liang, Chao Qi, Kun-Lin Yang and Jianzhong He: Bioresource Technology (2012).

Google Scholar

[2] Chieh-Lun Cheng, Pei-Yi Che, Bor-Yann Chen, Wen-Jhy Lee, Liang-Jung Chien and Jo-Shu Chang: Bioresource Technology 113 (2012) 58–64.

Google Scholar

[3] Manish Kumar, Kalyan Gayen: Applied Energy. (2012)88: 1999–(2012).

Google Scholar

[4] Thaddeus Ezeji, Caroline Milne, Nathan D. price, Hans P. Blaschek: Appl Microbiol Biotechnol (2010) 85: 1697-1712.

Google Scholar

[5] N. Qureshi, H.P. Blaschek: Renewable Energy 22 (2001) 557–564.

Google Scholar

[6] Hong-Wei Yen, Ruei-Jei Li, Te-Wei Ma: Journal of the Taiwan Institute of Chemical Engineers 42 (2011) 902–907.

Google Scholar

[7] Petra Patakova, Michaela Linhova, Mojmir Rychtera, Leona Paulova, Karel Melzoch. Biotechnology Advances. 31 (2013) 58–67.

DOI: 10.1016/j.biotechadv.2012.01.010

Google Scholar

[8] Oksana V. Berezina, Agnieszka Brandt, SergeyYarotsky, Wolfgang H. Schwarz, Vladimir V. Zverlov: Systimatic and Applied Microbiology 32 (2009) 449-459.

Google Scholar

[9] I. S. Maddox, N. Qureshi and K. Roberts-Thomson: ProcessBiochemistry Vol. 30, No. 3, pp.209-215. 1995 (1994).

Google Scholar

[10] H. A. George, J. L. Johnson, W. E. C. Moore, L. V. Holdeman and J. S. Chen: Appl. Environ. Microbiol. March 1983 vol. 45 no. 3 1160-1163.

Google Scholar

[11] Jui-Jen Chang, Chia-Hung Chou, Cheng-Yu Ho, Wei-En Chen, Jiunn-Jyi Lay and Chieh-Chen Huang: International Journal of Hydrogen Energy 33 (2008) 5I 37– 5I 46.

Google Scholar