Development of the Reliable Fluorometric Assay for the Sensitive Determination of Nitric Oxide Metabolites in Biological Fluids

Abstract:

In this study, we developed a fluorescence measurements in microplates for high-throughput determination of nitric oxide (NO) metabolites, which is used as a reliable and sensitive marker of NO production, in biological fluids using a pH-independent fluorescence increasing probe, DAMBO-P^H. The sensitivity of this method is lower than that of the other detection methods reported in the literature. This method was demonstrated to be useful for the rapid screening of large numbers of biological and clinical samples, and should be an important tool for further clarifying the physiology, pathology and pharmacology roles of NO.

• Periodical: Applied Mechanics and Materials (Volumes 477-478)
• Pages: 1359-1362
• DOI: https://doi.org/10.4028/www.scientific.net/AMM.477-478.1359
• Online since: December 2013
• Authors: Qiao Yang, Xiao Ling Zhang, Hong Liang Huang
• Keywords: Biological Fluids, High-Throughput, Microplate, NO Metabolites

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