Determination of Alkaline Phosphatase in Activated Sludge

Abstract:

Experiment disodium phenyl phosphate as a reaction basic, stromal hydrolysis produce phenol, the amount of phenol indirectly indicates the activity of Alkaline Phosphatase.Experiment by absorption wavelength, activated sludge volume, disodium phenyl phosphate, aluminum sulfate, reagent, temperature and other factors affecting the determination of Alkaline Phosphatase were studied.The experiments optimal reaction conditions initially identified. At 37°C, when activated sludge volume of 5mL, 15mL disodium phenyl phosphate absorbance measured at the maximum value, highest levels of Alkaline Phosphatase. Results show that, 0.25mL reagent optimum dosage for the trial, the best absorption wavelength through fitting the peak-valley is 590nm.

• Periodical: Applied Mechanics and Materials (Volumes 522-524)
• Pages: 643-647
• DOI: https://doi.org/10.4028/www.scientific.net/AMM.522-524.643
• Online since: February 2014
• Authors: Jun Yin, Yuan Ming Wang, Jian Hui Wang
• Keywords: Activated Sludge, Alkaline Phosphatase, Influencing Factor

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