Preparation and Characterization of Functionalized Magnetic Silica Nanospheres with the Immobilized Cellulase

Abstract:

Functionalized magnetic silica nanospheres were prepared by hydrolyzation of tetraethyl orthosilicate (TEOS) on Fe₃O₄ and modification of Fe₃O₄/SiO_x. Structure of functionalized magnetic silica nanospheres were characterized by transform infrared spectroscopy (FTIR) and differential thermal analysis (DTA). Cellulase was immobilized on functionalized magnetic silica nanospheres through cross-linking method. Saccharification rate of the immobilized cellulase is 70 % of that of free cellulase. The saccharification of the immobilized enzymes after 8 continuous uses still have 10 % of the initial saccharification rate, which demonstrate the potential of this immobilized cellulase for biofuel production.

• Periodical: Applied Mechanics and Materials (Volumes 543-547)
• Pages: 3892-3895
• DOI: https://doi.org/10.4028/www.scientific.net/AMM.543-547.3892
• Online since: March 2014
• Authors: Wen Juan Zhang, Jian Hui Qiu*, Hui Xia Feng, Xue Li Wu, Li Min Zang, Wei Yi, Sakai Eiichi
• Keywords: Cellulase, Functionalized, Immobilisation, Reusability

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