Orthogonal Experiment Selecting Extraction Process of Protodioscin in Trigonella Foenum-graecum l.

Abstract:

Objective: To select the best process extracting protodioscin from trigonella foenum-graecum L. Methods: Methods: Orthogonal experiment method is used, and the content of protodioscin is used as the indicator. And the influence of ethanol solution concentration, the ratio of material to solvent, extraction time and extraction times on extraction rate of protodioscin from trigonella foenum-graecum L. And the best extraction process is selected. Results: 75% of ethanol is used, the ratio of material to solvent is 1:60, and ultrasound extraction is made for three times, each time for 15min, which is the best extraction process. The experiment proves that the optimization conditions are reliable and are suitable for ultrasound extraction process of protodioscin in trigonella foenum-graecum L.

• Periodical: Applied Mechanics and Materials (Volumes 608-609)
• Pages: 1120-1127
• DOI: https://doi.org/10.4028/www.scientific.net/AMM.608-609.1120
• Online since: October 2014
• Authors: Qing Feng Guo*, Wei Wang
• Keywords: Orthogonal Experiment, Protodioscin, Trigonella Foenum-graecum L.

References

[1] China Pharmacopoeia Committee, Chinese Pharmacopeia [S], Beijing: Chemical Industry Press, 2005: 167.

[2] Jing Yu, Zhao Yuqing, Research progress of chemical components and pharmacological effect of Trigonel lafoenum graecum L. [J], Chinese Herbs, 2003, 34(12): 1146-1149.

[3] H iroshige H ibasam. Protodioscin isolated from fenugreek ( Trigonel lafoenum graecum L. induces cell death and m orphological change indicat ive of apoptosis in leukemic cell line H -60, but not in gastric cancercell line KATO III[J]. International Journal of Molecular Med icine, 2003, 11: 23.

DOI: 10.3892/ijmm.11.1.23

[4] Chen Huifang, Dictionary of active element of plant, the first edition [M], Beijing: China Medical Science Press, 2001: 789.

[5] Chen Jun, Liu Jihong, Research progress on vegetalitas traditional Chinese medicine promoting corpus cavernosum penis to be relaxed [J], National Journal of Andrology, 2003, 9(8): 615-617.

[6] Liu Xikui, Chen Guozhen, Yang Qingxiong, Research on activity of cardiovascular system of steroidal saponins [A], academic Annual Conference Proceedings of Chinese Pharmaceutical Association (Volume two) [C], Ningbo: 2000: 996.

[7] Nie Chun, Progress of anti-cancer effective components of natural medicine [J], Chinese Herb, 1999, 30(1) : 65-67.

[8] Mathematics department of Beijing University, Orthogonal experiment [M], Beijing: Petroleum Chemical Press, 1976: 166.

[9] Zhang Depei, Luo Yunling, Statistics of applied probability [M], Beijing: Higher Education Press, 2000: 167.