Research on the Extraction and Antibacterial Activity of Tea Polyphenols

Abstract:

There is an increasing interest in tea polyphenols, as natural plant compounds, in food products and also in pharmaceutical products due to their multiple health care functions. In this paper, a microwave-assisted extraction method was employed for the extraction of tea polyphenols. The effects of microwave power, heating time, and ethanol concentration on extraction of tea polyphenols were investigated by single factor experiments and an orthogonal experiment [L₉(3)³]. Results showed that the optimum extraction conditions were that the microwave power was 560W, the heating time was 30s, and the ethanol concentration was 40% (v/v). The antimicrobial properties of tea polyphenols were also discussed. Tea polyphenols had bacteriostatic activity against Escherichia coli and Staphylococcus aureus. This study may be valuable for the extraction and application of tea polyphenols.

• Periodical: Applied Mechanics and Materials (Volumes 644-650)
• Pages: 5157-5160
• DOI: https://doi.org/10.4028/www.scientific.net/AMM.644-650.5157
• Online since: September 2014
• Authors: Fei Long Sun*, Guo Ni Chen, Bing Wu, Wei Na Zhu, Yang Yan
• Keywords: Bacteriostatic Activity, Extraction, Tea Polyphenols

References

[1] Heiss M L, Heiss R J. The story of tea: a cultural history and drinking guide. [M]. Random House LLC, 2007, 31.

[2] Mukhtar H, Ahmad N. Tea polyphenols: prevention of cancer and optimizing health. [J]. The American Journal of Clinical Nutrition, 2000, 71(6): 1698-1702.

DOI: 10.1093/ajcn/71.6.1698s

[3] Heinrich U, Moore C E, De Spirt S, et al. Green tea polyphenols provide photoprotection, increase microcirculation, and modulate skin properties of women. [J]. The Journal of nutrition, 2011, 141(6): 1202-1208.

DOI: 10.3945/jn.110.136465

[4] Chen L, Lee M J, Li H, et al. Absorption, distribution, and elimination of tea polyphenols in rats. [J]. Drug Metabolism and Disposition, 1997, 25(9): 1045-1050.

[5] Sirun Y, Yan M, Hongjie Z. Application of Tea Polyphenols. [J]. Journal of Agriculture, 2011, 4: 44-48.

[6] Zhang Y, Zheng B, Tian Y, et al. Microwave-assisted extraction and anti-oxidation activity of polyphenols from lotus (Nelumbo nucifera Gaertn. ) seeds. [J]. Food Science and Biotechnology, 2012, 21(6): 1577-1584.

DOI: 10.1007/s10068-012-0210-2

[7] RenJie L. Orthogonal test design for optimization of the extraction of polysaccharides from Phascolosoma esulenta and evaluation of its immunity activity. [J]. Carbohydrate polymers, 2008, 73(4): 558-563.

DOI: 10.1016/j.carbpol.2007.12.026

[8] Qian L, Tao Y, Xie J. Antimicrobial mechanisms of tea polyphenols against Staphylococcus aureus and Pseudomonas aeruginosa. [J]. Microbiology, 2010, 37(11): 1628-1633 (in Chinese).

[9] Delazar A, Nahar L, Hamedeyazdan S, et al. Microwave-assisted extraction in natural products isolation. [M]. Natural Products Isolation. Humana Press, 2012: 89-115.

DOI: 10.1007/978-1-61779-624-1_5

[10] Teng H, Lee W Y. Optimization of microwave-assisted extraction of polyphenols from mulberry fruits (Morus alba L. ) using response surface methodology. [J]. Journal of the Korean Society for Applied Biological Chemistry, 2013, 56(3): 317-324.

DOI: 10.1007/s13765-012-3262-3

[11] Yi S, Wang W, Bai F, et al. Antimicrobial effect and membrane-active mechanism of tea polyphenols against Serratia marcescens. [J]. World Journal of Microbiology and Biotechnology, 2014, 30(2): 451-460.

DOI: 10.1007/s11274-013-1464-4

[12] Herrero M, Cifuentes A, Ibanez E. Sub-and supercritical fluid extraction of functional ingredients from different natural sources: Plants, food-by-products, algae and microalgae: A review. [J]. Food chemistry, 2006, 98(1): 136-148.

DOI: 10.1016/j.foodchem.2005.05.058