Temperature-Responsive Chromatographic System Using N-Isopropylacrylamide Polymer-Modified Columns

Wei Dong; Xu Fan; Guang Qun Cao; Wen Jia Zhu

doi:10.4028/www.scientific.net/AMM.651-653.124

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HomeApplied Mechanics and MaterialsApplied Mechanics and Materials Vols. 651-653Temperature-Responsive Chromatographic System...

Temperature-Responsive Chromatographic System Using N-Isopropylacrylamide Polymer-Modified Columns

Abstract:

Temperature-responsive poly (N-isopropylacrylamide) (NIPAM_n) was grafted onto porous silica (Sil-NIPAM_n) to be used as stationary phase for normal-phase high performance liquid chromatography (NP-HPLC). Specific interaction ability and unique selectivity were detected between NIPAM_n and polyaromatic hydrocarbons (PAHs) through monitoring the retention time with increasing the temperature in NP-HPLC process. The results showed that Sil-NIPAM_n has better retention behaviors for structural isomers of PAHs when the temperature is above its lower critical solution temperature (LCST) in mixture of n-hexane/isopropanol. It indicates that NIPAM_ngrafted on the silica shows phase transition from random coil to stretch state to increase interaction between solutes and stationary phase when temperature is above its LCST.