Image Recognition and Counting for the Bacilli Cell Based on the Microscopic Image

Article Preview

Abstract:

In order to automatically detect bacilli in sputum image with microscopy, an intelligent recognition method based on machine vision is presented. Firstly, a novel background filter was designed based on the single layer perceptron to realize object segmentation from background. After eliminating the short twig and small area noise, the suspicious goals and the image noise are separated. In the feature extraction, besides the base features of single bacillus two important features are presented to solve the difficult problem of identification and counting for the overlapping and winding bacilli cells. Finally, an EBP neural network classifier is designed for the accurate identification and counting of the bacilli cells. Experimental results verified the effectiveness of the presented method.

You might also be interested in these eBooks

Info:

Periodical:

Pages:

2318-2321

Citation:

Online since:

September 2014

Export:

Price:

Permissions CCC:

Permissions PLS:

Сopyright:

© 2014 Trans Tech Publications Ltd. All Rights Reserved

Share:

Citation:

* - Corresponding Author

[1] K. Veropoulos, G. Learmonth, C. Campbell, B. Knight, J. Simpson, Automatic identification of tubercle bacilli in sputum. A preliminary investigation, Analytical and Quantitative Cytology and Histology, 21(4), 277–81 (1999).

Google Scholar

[2] M. G. Forero, F. Sroubek, G. Cristóbal, Identification of tuberculosis bacteria based on shape and color, Real-Time Imaging. 10, 251–262 (2004).

DOI: 10.1016/j.rti.2004.05.007

Google Scholar

[3] M. Wilkinson, Rapid automatic segmentation of fluorescent and phase-contrast images of bacteria. In: Slavik J, editor. Fluores-cence microscopy and fluorescent probes, New York, NY: Plenum Press (1996).

DOI: 10.1007/978-1-4899-1866-6_40

Google Scholar

[4] M. G. Forero, G. Cristóbal and M. Desco, Automatic identification of Mycobacterium tuberculosis by Gaussian mixture models, Journal of Microscopy. 223, 120–132 (2006).

DOI: 10.1111/j.1365-2818.2006.01610.x

Google Scholar

[5] J. Mitchell, A Geometric Interpretation of Hidden Layer Units in Feedforward Neural Networks, Neural Networks. 3, 19- 25 (1992).

DOI: 10.1088/0954-898x_3_1_004

Google Scholar