Development of a SPE-HPLC-PDA Method for the Determination of Tetracyclines in Soils

Abstract:

A simple and rapid SPE-HPLC/PDA method has been developed for the quantitative determination of tetracyclines in soils. An ultrasonic extraction system for the soil samples was confirmed as a combination of a 1:2 ratio of EDTA-McIlvaine buffer solution with methanol by comparing the extraction efficiency of 4 solvents and optimising the combination ratio. The soil extracts were purified and concentrated by Oasis-MAX cartridge followed by HPLC separation and PDA detection. The optimal chromatographic condition effectively separated the object and interfering substance, which can improve the sensitivity of the instrument. The limits of detection were lower than 20 μg·kg^-1, and the limits of quantification were no more than 60 μg·kg^-1 for all compounds in the soil. The recoveries of tetracyclines peaked at levels of 50-500 μg·kg^-1 and averaged at 62.1% to 108.9% with relative standard deviation values of less than 5%. This method was applied to analyse real soil samples from suburban Beijing farmland, and TC and OTC residues were detected in the soil.

• Periodical: Applied Mechanics and Materials (Volumes 675-677)
• Pages: 288-294
• DOI: https://doi.org/10.4028/www.scientific.net/AMM.675-677.288
• Online since: October 2014
• Authors: Bao Li Sun*, Yi Wei Dong, Hong Shan, Jin Li Huang, Cheng Feng Tong
• Keywords: HPLC/PDA, MAX, Soil, SPE, Tetracyclines (TCs)

References

[1] Halling-Sarensen, B., Nielsen, S.N., Lanzky, P.F., Ingersle, F., Lutzhaft, H.C.H., Jargensen, S.E. Chemosphere , Vol. 36(1998), pp.357-394.

[2] Kemper, N., Ecology Indicator. Vol. 8(2008), p.1–13.

[3] Hou Fangliang, in: Feed additive application, edited by China agriculture press. Beijing (2003), in press.

[4] Li Shuyi, Zheng huidian, Mu xinrong. Ecology and Environment, Vol. 14(2005), pp.266-270.

[5] Shi Yi, Zhao Muqiu, Wang Jun, Song Yufang. Journal of Agro-Environment Science, Vol. 29(2010), pp.240-224.

[6] Wu Qingfeng, Hong Hanlie, Li Zhaohui. Safety and Environmental Engineering, Vol. 17(2010), pp.68-72.

[7] Sarmah , Ajit K., Michael T. Meyer, Alistair B.A. Boxall. Chemosphere, Vol. 65(2006), p.725–759.

[8] Munir M, Xagoraraki. J Environment Quality, Vol. 40(2011), p.248–255.

[9] Montforts, M.H.M.M. in: Environmental risk assessment for veterinary medicinal products, edited by National Institute of Public Health and the Environment, Bithoven, The Netherlands(1999), in press.

[10] Hamscher, G., Sczesny, S., Hoü per, Nau, H. Anal. Chem. Vol. 74(2008), p.1509–1518.

[11] De Liguoro, M., Cibin, V., Capolongo, F., Halling-SÖensen, B., Montesissa, C. Chemosphere, Vol. 52(2003), p.203–212.

DOI: 10.1016/s0045-6535(03)00284-4

[12] Halling-SÖensen, B., SengelÖ, G., TjÖnelund, J. Arch. Environ. Contam. Toxicol. , Vol. 44(2002), p.7–16.

[13] Kumar, K., Gupta, S.C., Chander, Y., Singh, A. K Adv. Agro. Vol. 87(2002), p.1–54.

[14] Hirsch, R., Ternes, T., Haberer, K., Kratz, K.L. Sci. Total Environ. Vol. 225(1999), p.109–118.

[15] Li-Jun Zhou, Guang-Guo Ying , Shan Liu. Journal of Chromatography A. Vol. 1244(2012), p.123– 138.

[16] ZHENG Wenli, ZHANG Lifang, ZHANG Keyu, WANG Xiaoyang and XUE Feiqun. Journal of Integrative Agriculture. Vol. 11 (2012), pp.1189-1198.

[17] Ádám Tölgyesi, László Tölgyesi, Katalin Békési, Virender K. Sharma, Jenő Fekete. Meat Science. Vol. 96 (2014). p.1332–1339.

DOI: 10.1016/j.meatsci.2013.11.011

[18] Blackwell, P.A., Holten LüzhÖft, H. -C., Halling-SÖensen H. -P. Talanta. Vol. 64 (2004) p.1058.

[19] Yang J.F., Ying G.G., Zhao J.L. et al. Chen, Sci. Total Environ. Vol. 408(2010), pp.424-3432.

[20] Kim S.C., Carlson K. Anal. Bioanal. Chem. Vol. 387 (2007) , pp.1301-3015.

[21] Andreu V., Vazquez-Roig P., Blasco C. , Pico Y. Anal. Bioanal. Chem. Vol. 394 (2009), pp.1329-1339.

[22] Jacobsen A.M., Halling-Sorensen B., Ingerslev F. Chromatogr. A. Vol. 1038(2004), pp.157-170.

[23] JacobsenA.M. , Halling-Sorensen B. Anal. Bioanal. Chem. Vol. 384(2006), pp.1164-1174.

[24] Lillenberg M., Yurchenko S., Kipper K. Chromatogr. A Vol. 1216(2009), pp.5949-5954.

[25] Vazquez-Roig P., Segarra R., Blasco C. Chromatogr. A Vol. 1217(2010), pp.2471-2483.

[26] GB/T 18932. 4- 2002《Method for the determination of oxytetracycline, tetracycline, chlortetracycline, and doxycycline residues in honey-Liquid chromatog aphic method》.