Detection of the Antibiotic TMP in the Water with HPLC-DAD

Xiao Na Ji; Dong Mei Wang; Zhi Min Ren; Hua Yang; Yan Bo Xiao; De Jun Bian

doi:10.4028/www.scientific.net/AMM.727-728.790

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HomeApplied Mechanics and MaterialsApplied Mechanics and Materials Vols. 727-728Detection of the Antibiotic TMP in the Water with...

Detection of the Antibiotic TMP in the Water with HPLC-DAD

Abstract:

Thispaper studies the method of detecting antibiotic trimethoprim (TMP) in theenvironment water, sewage and tap water with high performance liquidchromatography –diode array detector (HPLC-DAD). The chromatographic conditionis confirmed that: the stationary phase is ODS, the mobile phase is acetonitrileand methanoic acid aqueous solution of 0.1%（12:88）, the flow rate is 1.5min·L^-1; theinjection volume is 5μL，the chromatographic column temperature is 25°C,DAD detection wavelength is 272nm. The experiment result shows that, the linearlydependent coefficient of external standard curve is 0.996, the detection limitis 0.017 mg·L^-1, adding standard recovery ranges from 92.6-140.6 ％.

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Periodical:

Applied Mechanics and Materials (Volumes 727-728)

Pages:

790-793

DOI:

https://doi.org/10.4028/www.scientific.net/AMM.727-728.790

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Online since:

January 2015

Authors:

Xiao Na Ji, Dong Mei Wang, Zhi Min Ren, Hua Yang, Yan Bo Xiao, De Jun Bian

Keywords:

Antibiotic, HPLC, Trimethoprim (TMP), Water

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