Phytochemistry and Antioxidant Activity of Molineria latifolia Herb. Extracts

Abstract:

Molineria latifolia is a perennial-monocotyle-donous herb and it is well-known in the treatment of melasma, freckle and black spot in Thai traditional medicine. The purpose of this study was to determine the phytochemicals and antioxidant activity in the aerial parts and the underground parts of M. latifolia by water, ethanolic and ethyl acetate extracts. The phytochemicals were analyzed by HPTLC. The antioxidant activity was measured by using the DPPH radical scavenging activity assay, Ferric reducing antioxidant power assay and Total phenolic content was measured by Folin‑Ciocalteu. The result showed the highest antioxidant activity of the underground parts (RW) were extracted by water at EC₅₀=245.03±6.59 µg/mL(DPPH) and 640.03±0.36 Fe²⁺/ mg (FRAP). Consequently, the highest phenolics content was found in the water extract of the aerial parts (LW) (191.70±1.64 µg FA/mg). M. latifolia extracts contain a bioactive compound of Tannin, Flavonoids and Steroid . Tannin and Flavonoid were found in both of parts. In the aerial parts were found steroid extracting by ethyl acetate and ethanolic, as the underground parts were seen steroid extracting by water. It was found that M. latifolia has high bioactive compounds and moderate antioxidant activity. These results for the cosmetic product development in the future.

• Periodical: Applied Mechanics and Materials (Volume 891)
• Pages: 21-29
• DOI: https://doi.org/10.4028/www.scientific.net/AMM.891.21
• Online since: May 2019
• Authors: Kunthasaya Akkarasiritharattana*, Siriphatr Chamutpong
• Keywords: Antioxidant Activity, Molineria latifolia, Phytochemistry

References

[1] K.A. Gupta, D.M. Gover, K. Nouri, S. Taylor, The treatment of melasma: A review of clinical trials, J. Am. Acad. Derm.7 (1981) 698–710.

[2] K.A. Gupta, D.M. Gover, K. Nouri, S. Taylor, The treatment of melasma: A review of clinical trials, J. Am. Acad. Derm. (2006) 1048–1065.

[3] A. Kocyan, D.A. Snijman, F. Forest, D.S. Devey, J.V. Freudenstein, J. Wiland-Szymańska, M.W. Chase, P.J. Rudall, Molecular phylogenetics of Hypoxidaceae-evidence from plastid DNA data and inferences on morphology and biogeography, Mol Phylogenet Evol. 60:1 (2011) 122-136.

DOI: 10.1016/j.ympev.2011.02.021

[4] J. Kulip, An ethnobotanical survey of medicinal and other useful plants of Muruts in Sabah, Malaysia, Telopea. 10:1 (2003) 81-98.

DOI: 10.7751/telopea20035608

[5] E. Norhajar, A.K. Kamziah, M. Nazre, A.G. Awang, Medicinal Plant Diversity and Vegetation Analysis of Logged over Hill Forest of Tekai Tembeling Forest Reserve, Jerantut, Pahang, J Agric Sci. 2:3 (2010) 189-210.

DOI: 10.5539/jas.v2n3p189

[6] Y. Kurihara, S. Nirasawa, Structures and activities of sweetness-inducing substances (miraculin, curculin, strogin) and the heat-stable sweet protein, mabinlin, Foods and Food Ingredients J. Jpn. (1997) 67-74.

[7] E. Kurimoto, E. Amemiya, K. Kato, Structural Biology of Curculin, a Taste-Modifying Protein, Foods Food Ingredients J. Jpn. 213:7 (2008) 645-652.

[8] K. Macek, Pharmaceutical applications of thin-layer and paper chromatography, third ed., Medical Department Medical Faculty, Charles University Prague, Czechoslovakia, (1972).

[9] H. Wagner, S. Bladt, Plant drung analysis A Thin Layer Chromatography Atlas, Second ed., Michigan, Germany, (1996).

[10] K. Schlesier, M. Harwat, V. Bohm, R. Bitsch, Assessment of antioxidant activity by using different in vitro methods, Free Radic. Res. 36:2 (2002) 177-187.

DOI: 10.1080/10715760290006411

[11] I. F. Benzie, J. J. Strain, Ferric reducing/antioxidant power assay: direct measure of total antioxidant activity of biological fluids and modified version for simultaneous measurement of total antioxidant power and ascorbic acid concentration, Meth. Enzymol. 299 (1999) 15-17.

DOI: 10.1016/s0076-6879(99)99005-5

[12] V.L. Singleton, J.A. Rossi, Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagent, Am J Enol Vitic. 16 (1965) 144-158.