Optimization Statistical Condition of Incubation for the Growth State of a Recombinant Strain Produced Alkaline Protease

Mei Shuo Zhang; Yan An Yang; Jing Bo Liu; Song Yi Lin

doi:10.4028/www.scientific.net/AMR.1025-1026.482

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HomeAdvanced Materials ResearchAdvanced Materials Research Vols. 1025-1026Optimization Statistical Condition of Incubation...

Optimization Statistical Condition of Incubation for the Growth State of a Recombinant Strain Produced Alkaline Protease

Abstract:

Recombinant E. coli strain was used to yield alkaline protease. The effects of components of medium and condition of incubation for the growth state of a recombinant strain including glucose concentration, peptone concentration, volume of salt solution, incubation time and agitation frequency on density of recombinant strain were optimized using one-factor-at-a-time experiment. The optimal components of medium were glucose concentration of 8 g/L, peptone concentration of 6 g/L and volume of salt solution 10 mL. And condition of incubation was obtained at agitation frequency of 210 r/min, keep it at 37°C for 32 h.

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Periodical:

Advanced Materials Research (Volumes 1025-1026)

Pages:

482-486

DOI:

https://doi.org/10.4028/www.scientific.net/AMR.1025-1026.482

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Online since:

September 2014

Authors:

Mei Shuo Zhang, Yan An Yang, Jing Bo Liu, Song Yi Lin*

Keywords:

Alkaline Protease, Growth State, Optimization, Recombinant Strain

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