An Host-Guest Electrochemical Sensor for DNA Ligase Detection

Wei Xiong; Yan Hua Ding; Xiu Xiu Zhang; Hao Fan

doi:10.4028/www.scientific.net/AMR.1033-1034.427

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HomeAdvanced Materials ResearchAdvanced Materials Research Vols. 1033-1034An Host-Guest Electrochemical Sensor for DNA...

An Host-Guest Electrochemical Sensor for DNA Ligase Detection

Abstract:

We herein constructed a electrochemial sensor for T4 DNA ligase detction. In the sensor, the hairpin dsDNA terminal-labeled with 4-((4-(dimethylamino)phenyl)azo)benzoic acid (dabcyl) and thiol group was immobilized on Au electrode surface as the probe by Au-S bond, and the CdS nanoparticles surface-modified with β-cyclodextrins (ZnS-CD) were employed as electrochemical signal provider and host-guest recognition element. After T4 DNA ligase repairing single-strand breaks in duplex DNA, the dsDNA probe underwent a significant conformational change, which forced dabcyl away from the electrode. As a result, formerly shielded dabcyl became accessible to host-guest recognition between β-cyclodextrin and dabcyl, thus the target repairing event could be sensitively transduced to electrochemical signal provided by ZnS-CD.

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Periodical:

Advanced Materials Research (Volumes 1033-1034)

Pages:

427-430

DOI:

https://doi.org/10.4028/www.scientific.net/AMR.1033-1034.427

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Online since:

October 2014

Authors:

Wei Xiong, Yan Hua Ding, Xiu Xiu Zhang, Hao Fan*

Keywords:

DNA Ligase, Electrochemical Sensors, Host-Gust, Nanoparticle

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