Advanced Materials Research Vol. 1058

Abstract: To explore the effect of Norcantharidin on the biological behavior of SW579 cells. The method of MTT is used to detect the inhibition of Norcantharidin with different concentration on the proliferation of SW579 cells. Transwll assay detects the inhibition of cantharidin on the invasiveness of SW579 cells. And ELISA assay detects the related invasion protein MMP2. ELISA method is used to detect VEGF expression level, and to study the inhibition of cantharidin on the angiogenesis. MTT results indicate that cantharidin has inhibition effects on the proliferation of SW579 cell, and with the increase of concentration, the inhibition effects are enhanced. Transwll assay and ELISA assay indicates that cantharidin has inhibition effects on the invasiveness of SW579 cell. The results of VEGF suggest that cantharidin has inhibition effects on angiogenesis.

Abstract: In vitro rapid cell-based bioassay is one of the effective methods to evaluate cardio-myocyte function by characteristics of beating rate, contractility, and toxicity. In this study, rapid profile assessing drug-induced arrhythmia was studied by treating cardiomyocyte-based biosensor with some drugs, which resulted in compound-specific changes in the cardiomyocyte beating evolution profiles and growth evolution profiles. Also, rapid profile assessment of cardiomyocyte-based biosensor was also determined by several types of compoundsFrom the compound experiment results, cardiomyocyte-based biosensor with real-time cell analysis technology can monitor the cardiomyocyte beating status in a non-invasive way and indicate the potential of drug-induced arrhythmia.