Cholic Acid-Modified Polyethylenimine for Gene Delivery into Human Carcinoma Cells

Abstract:

Polyethylenimine (PEI) was modified by cholic acid at a molar ratio of 1:1. Cholic acid (CA)-modified PEI (PEI-CA) were evaluated for formation of DNA complexes. PEI-CA/pEGFP plasmid DNA complexes were characterized for their size and zeta potential. Gel electrophoresis showed total retardation for PEI-CA/pEGFP complexes formed at weight ratios above 0.25. The particle size and zeta potential of the complexes at a polymer-to-DNA ratio of 0.5 were 295.3 nm and 30.5 mV, respectively. The transfection efficiency of PEI-CA/pEGFP complexes was comparable to unmodified PEI. Cytotoxicity result showed that PEI-CA had lower cytoxicity than PEI. This study suggests that PEI-CA has potential utility as a gene delivery carrier.

• Periodical: Advanced Materials Research (Volume 1060)
• Pages: 3-6
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.1060.3
• Online since: December 2014
• Authors: Wanlop Weecharangsan*, Orapan Paecharoenchai, Nattisa Ni-yomtham, Praneet Opanasopit, Boon Ek Yingyongnarongkul, Robert J. Lee
• Keywords: Cholic Acid, Gene Delivery, Polyethylenimine

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