Isolation and Characterization of the Algicidal Bacterium DCJ-2

Abstract:

Algicidal bacteria as part of the microbial populations in natural waters, is possible toinhibit the growth of algae or kill algae, dissolve algal cells in a direct or indirect way. In this study,four algicidal bacteria were isolated from cyanobacteria bloom water of Lake Dianchi, designated asDCJ-1, DCJ-2, DCJ-3, DCJ-4 respectively. The algicidal range and algicidal activity assay of the fouralgicidal bacteria showed that they have different algicidal range on the 10 tested algal species, whichstrain DCJ-2 has the best algicidal activity. The results indicated that bacterial strain DCJ-2 has highalgicidal activity against the Plectonema boryanum and exhibited algicidal activity through directattack. The lytic effect of strain DCJ-2 against Plectonema boryanum was time-dependent. It could be a potential bio-agent to control the blooms of cyanobacteria.

• Periodical: Advanced Materials Research (Volumes 1065-1069)
• Pages: 3077-3082
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.1065-1069.3077
• Online since: December 2014
• Authors: Ting Ting Liu, Yu Fu Peng, Li Liu*, Xue Shan Xia
• Keywords: Algicidal Activity, Algicidal Bacteria, Biological Control, Cyanobacteria Bloom, Red Tides

References

[1] Anderson DM, Cembella AD, Hallegraeff GM: Progress in understanding harmful algal blooms: paradigm shifts and new technologies for research, monitoring, and management. Ann Rev Mar Sci 4: 143-176. (2012).

DOI: 10.1146/annurev-marine-120308-081121

[2] Van Dolah FM: Marine algal toxins: origins, health effects, and their increased occurrence. Environ Health Perspect 108: 133-141. (2000).

DOI: 10.1289/ehp.00108s1133

[3] Seong-Cheol Park, Jong-Kook Lee, Si Wouk Kim, Yoonkyung Park: Selective Algicidal Action of Peptides against Harmful Algal Bloom Species. PLoS One 10: e26733. (2011).

DOI: 10.1371/journal.pone.0026733

[4] Jianfei Luo, Yuan Wang, Shuishui Tang, et al: Isolation and Identification of Algicidal Compound from Streptomyces and Algicidal Mechanism to Microcystis aeruginosa. PLoS One10: e76444. (2013).

DOI: 10.1371/journal.pone.0076444

[5] Lee YJ, Choi JK, Kim EK, Youn SH, Yang EJ: Field experiments on mitigation of harmful algal blooms using a sophorolipid-yellow clay mixture and effects on marine plankton. Harmful Algae 7: 154-162. (2008).

DOI: 10.1016/j.hal.2007.06.004

[6] Kodama M, Doucette GJ, Green DH: Relationships between bacteria and harmful algae. In: E. Grane, li and J T. Turner, editors. Ecology of Harmful Algae Berlin: Springer. Pp. 243-255. (2006).

DOI: 10.1007/978-3-540-32210-8_19

[7] Imai I, Ishida Y, Hata Y, et al: Isolation of a marine gliding bacterium that can kills Chattonella Antiqua (Raphidophyceac) [J]. Nibon Suisan Gakkai-shi, 57: 1409. (1991).

DOI: 10.2331/suisan.57.1409

[8] Azam F: Microbial control of oceanic carbon flux: The plot thickens. Science 280: 694-696. doi: 10. 1126/science. 280. 5364. 694. (1998).

DOI: 10.1126/science.280.5364.694

[9] Jeong H, Yim J H: Genomic blueprint of Hahella chejuensis, a marine microbe producing an algicidal agent. Nucleic Acids Res, 33 (22): 7066-7073. (2005).

DOI: 10.1093/nar/gki1016

[10] Anderson D M. Approaches to monitoring, control and management of harmful algal blooms (HABs). Ocean Coast Manage, 52: 342-347. (2009).

DOI: 10.1016/j.ocecoaman.2009.04.006

[11] Lovejoy C, Bowman J P, et al: Algicidal effects of a novel marine Pseudoalteromonas Isolate (class Proteobacteria, gamma subdivision) on harmful Algal bloom species of the Genera Chattonella, Gymnodinium, and Heterosigma [J]. Appl Environ Microbiol, 64 (8): 2806-2813. (1998).

DOI: 10.1128/aem.64.8.2806-2813.1998

[12] Amin SA, Parker MS, Armbrust EV: Interactions between diatoms and bacteria. Microbiol Mol Biol Rev 76: 667-684. (2012).

DOI: 10.1128/mmbr.00007-12

[13] Fraleigh P C, Burnham J C: Myxococcal predation on cyanobacterial populations: nutrient effects [J]. Limnol Oceanogr, 3 (3): 476-483. (1988).

DOI: 10.4319/lo.1988.33.3.0476

[14] Mu RM, Fan ZQ, Pei HY, Yuan XL, Liu SX et al: Isolation and algae-lysing characteristics of the algicidal bacterium B5. J Environ Sci China 19: 1336-1340. doi: 10. 1016/S 1001-0742 (07) 60218-6. PubMed: 18232228. (2007).

DOI: 10.1016/s1001-0742(07)60218-6

[15] Imai I, Ishida Y, Hata Y: Killing of marine phytoplankton by a gliding bacterium Cytophga sp. Isolated from the coastal sea of Japan [J]. Mar Biol, 116: 527-532. (1993).

DOI: 10.1007/bf00355470

[16] Anderson DM: Approaches to monitoring, control and management of harmful algal blooms (HABs). Ocean Coast Manag 52: 342-347. (2009).

DOI: 10.1016/j.ocecoaman.2009.04.006

[17] Yamamoto Y, Kouchiwa T, Hodoki Y, Hotta K, Uchida H et al: Distribution and identification of actinomycetes lysing cyanobacteria in a eutrophic lake. J Appl Phycol 10: 391-397. doi: 10. 1023/A: 1008077414808. (1998).

DOI: 10.1023/a:1008077414808

[18] Yang JD, Hu LB, Zhou W, Yin YF, Chen J, et al: Lysis of Microcystis aeruginosa with extracts from Chinese medicinal herbs. Int J Mol Sci 10: 4157-4167. doi: 10. 3390/ijms 10094157. PubMed: 19865537. (2009).

DOI: 10.3390/ijms10094157

[19] Safferman RS, Morris ME: Algal virus: Isolation. Science 140: 679-680. doi: 10. 1126/science. 140. 3567. 679. PubMed: 13976026. (1963).

DOI: 10.1126/science.140.3567.679