The Investigation of Biosensor for Tetrodotoxin Monitoring

Abstract:

Using Bacillus licheniformis as identify element, and it was immobilized by sandwich immobilization, a biosensor was fabricated based on oxygen electrode. The experiment indicated that the appropriate pH range is 7.76, the optimum temperature is 35±0.5 °C, and the appropriate concentration of GGA is 21.5 mg/L in bottom fluid, toxicity sensor of biosensor based on Bacillus licheniformis has a linear response from 10 to 80 μg/L and the standary curve is y=0.0026x-0.01, its linear correlation is 0.9669. The immobilization membrane produced by Bacillus licheniformi save more than 40 days, and measured 3 ~ 4d for activation, the performance of the system is stable.

• Periodical: Advanced Materials Research (Volumes 1073-1076)
• Pages: 584-587
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.1073-1076.584
• Online since: December 2014
• Authors: Jing Chen, Zhuo Yang*
• Keywords: Bacillus Licheniformis, Biosensor, Reviving, Saving, Tetrodotoxin

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