Study on Preparation of Glucose by Poplar Cellulose Enzymatic Hydrolysis

Zhan Song Ma; Yu Liu; Zhen Wang

doi:10.4028/www.scientific.net/AMR.1081.25

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HomeAdvanced Materials ResearchAdvanced Materials Research Vol. 1081Study on Preparation of Glucose by Poplar...

Study on Preparation of Glucose by Poplar Cellulose Enzymatic Hydrolysis

Abstract:

This paper made the detailed discourse for the study on using cellulase, hemicellulase hydrolysis of poplar fiber raw material to make glucose. The raw material used in this experiment is poplar, cellulase and hemicellulase enzymes are used. Hemicellulase is a complex system, xylose is regarded as the main chain for a lot of hemicellulose. The experiment used dry poplar as raw material, through the ball mill, ground poplar into wood powder. In the experiment, the optimal condition of enzyme hydrolysis of wood powder has been discussed based on reaction temperature, reaction time, enzyme concentration, and the pH value of reaction. Finally, under the optimal condition, use enzyme to hydrolyse wood powder, measure reducing sugar content by the DNS method, and calculate the rate of hydrolysis.

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Periodical:

Advanced Materials Research (Volume 1081)

Pages:

25-30

DOI:

https://doi.org/10.4028/www.scientific.net/AMR.1081.25

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Online since:

December 2014

Authors:

Zhan Song Ma*, Yu Liu, Zhen Wang

Keywords:

Air Drying Cypress, Ball Mill, Cellulase, DNS Method, Glucose, Hemicellulase

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* - Corresponding Author

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