RP-HPLC-UV Method for Simultaneous Determination of Isoflavonoids in Huangqi Gegen and its Markted Formulations

Abstract:

A new simple and efficient RP-HPLC-UV method was developed for the simultaneous analysis of isoflavonoids in Huangqi Gegen and its marketed formulations. Effective chromatographic separation of ten active components was achieved on a Kromasil C₁₈ column (4.6×250 mm, i.d.; 5 μm) with gradient elution of methanol and 0.2% formic acid aqueous solution at a flow rate of 1.0 mL/min in 60 min. Detection was performed at 250nm. The validation of the method included tests of linearity, sensitivity, precision, repeatability, stability and accuracy. All calibration curves showed good linear regression (r>0.999) within test ranges. The established method showed good precision and accuracy with overall intra-day and inter-day variations of 0.77-1.81 % and 1.38-2.47 %, and overall recoveries of 96.64-103.96 % for the compounds analyzed. The developed method was successfully applied for the quantitative analysis of constituents in Huangqi gegen and its marketed formulations.

• Periodical: Advanced Materials Research (Volume 1083)
• Pages: 84-89
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.1083.84
• Online since: January 2015
• Authors: Wen Liang Lu, Zhi Ping Wang*, Wei Min Li, Yi Fei Wang, Yan Jun Zhang
• Keywords: Gegen, HPLC, Huangqi, Isoflavonoids, Marketed Formulations

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