The Progress in Cloning, Expression and Purification of Cholinesterase in Pichia pastoris: One Kind of Biomaterial for the Detection of Residual Insecticide Contamination

Abstract:

Cholinesterase serves as a key enzyme of the cholinergic system, which could be one kind of promising biomaterial to assay OP and CB residues. However, cholinesterase purified from tissues or blood is both time and cost intensive. In this essay, different methods via biotechnology to get this biomaterial in Pichia pastoris is summarized, including cholinesterase gene cloning and expression vector construction, construction of genetic modified yeast, screening high yield transformant, purification from supernatant and activity analysis.

• Periodical: Advanced Materials Research (Volumes 1120-1121)
• Pages: 847-852
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.1120-1121.847
• Online since: July 2015
• Authors: Jing Jing Tian, Yuan Hong Xie, Xiang Ning Chen*, Xi Yue, Zhen Long Guo, Wen Tao Xu
• Keywords: Biomaterial, Biotechnology, Cholinesterase, Expression, Pichia pastoris

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