Neutrophilic Microbial Community with High Rate of Elemental Sulfur Oxidation

Abstract:

Using molecular genetic methods, 10 species wereidentified in the community of neutrophilic microorganisms isolated from thebioreactor during the process of biooxidation of gold-arsenicpyrrhotite-bearing sulfide ore flotation concentrate. The microbial communitywas composed of chemolithotrophs oxidizing elemental sulfur and its reducedcompounds and chemoorganotrophs. The predominant S⁰-oxidizingchemolithotrophic strain and 3 strains of chemoorganotrophs were isolated inpure cultures. Phylogenetic identification of the pure cultures revealed thechemolithotrophic microorganism to belong to Thermithiobacillus tepidarius,while organotrophic microorganisms were identified as Parapedobacter sp.,Nocardioides nitrophenolicus, and Nocardioides sp. Themorphological and physiological characteristics of Thermithiobacillustepidarius and Nocardioides nitrophenolicus, the culturespredominant in the community of neutrophilic microorganisms, were studied.Active growth of T. tepidarius occurs within a temperature range of38–48°C at the optimal pH value of 7.0–7.5. The maximal specific growth rate ofT. tepidarius on sulfur and on solid residue which remains after theprocess of bioleaching/biooxidation of sulfide ore flotation concentrate (biooxidationresidue) was 0.13 and 0.135 h^-1, respectively. The average maximalrate of S⁰ oxidation in the biooxidation residue was 0.107 g S⁰_oxidizedper g S⁰_initial h^-1. The ability of Nocardioidesnitrophenolicus to destruct thiocyanate was shown.

• Periodical: Advanced Materials Research (Volume 1130)
• Pages: 59-62
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.1130.59
• Online since: November 2015
• Authors: A.V. Belyi*, Tamara F. Kondrat'eva, Aleksandr Bulaev, N.V. Solopova, N.V. Grigor’eva, M.I. Murav’yov, A.E. Panyushkina, I.A. Tsaplina
• Keywords: Elemental Sulphur, Molecular Genetic Research Methods, Neutrophilic Microbial Community, Species, Strain Composition, Thiocyanate

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