Effects of Fe²⁺ on Fermentation and Biohydrogen Production of Biohydrogenbacterium R3 sp.nov.

Abstract:

This study investigated the effects of Fe2+ on the cell growth and hydrogen production to Biohydrogenbacterium R3 sp.nov. in the batch test. The hydrogen production got the maximum of 12.54mmol/L when the concentration of Fe2+ was 40 mg/L and the hydrogen content, OD600nm, glucose consumption and the concentration of ethanol had the maximum of 45.2 %, 1.31 g/L, 92 % and 1073.34 mg/L respectively when the concentration of Fe2+ was 100 mg/L. The phenomenon of feed back appeared when the concentration of Fe2+ exceeded 100 mg/L.

• Periodical: Advanced Materials Research (Volumes 152-153)
• Pages: 1062-1065
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.152-153.1062
• Online since: October 2010
• Authors: Yong Feng Li, Hong Chen, Tao Zhao, Xiao Jing Xiong, Xin Yao, Wei Han, Pin Hua Rao, Jing Li Xu
• Keywords: Biohydrogenbacterium R3 Sp.nov, Fe²⁺, Hydrogen Production

References

[1] Hansel A, Lindblad P. Towards optimization of cyanobacteria as biotechnologically relevant producers of molecular hydrogen, a clean and renewable energy source[J]. Appl Microbiol Biotechnol , 1998, 50: 153~160.

DOI: 10.1007/s002530051270

[2] Nan-Qi Ren, Dong-Yang Wang, Chuan-Ping Yang, Lu Wang, ing-Li Xu, Yong-Feng Li, Selection and isolation of hydrogen-producing fermentative bacteria with high yield and rate and its bioaugmentation process, International Journal of Hydrogen Energy, 2010(35) 2877~2882.

DOI: 10.1016/j.ijhydene.2009.05.040

[3] Yong Feng Li, Nan Qi Ren, Ying Chen, Guo Xiang Zheng, Ecological mechanism of fermentative hydrogen production by bacteria, International Journal of Hydrogen Energy 2007 (32) 755~760.

DOI: 10.1016/j.ijhydene.2006.08.004

[4] Fang HHP, Liu H. Effect of pH hydrogen production from glucose by a mixed culture. Bioresource Technol 2002; 82: 87~93.

DOI: 10.1016/s0960-8524(01)00110-9

[5] Voolapalli RK, Stuckey DC. Hydrogen production in anaerobic reactors during shock loads-influence of formate production and H2 kinetics. WatRes 2001; 35: 1831~1841.

DOI: 10.1016/s0043-1354(00)00441-3

[6] Dabrock B, Bahl H, Gottschalk G. Parameters affecting solvent production by Clostridium pasteurianum. Appl Environ Microbiol 1992; 58: 1233~1239.

DOI: 10.1128/aem.58.4.1233-1239.1992

[7] Debabrata Das, Vezirogu T Nejat. Hydrogen production by biological processes: a survey of literature[J]. Hydrogen Energy , 2001, 26: 13~28.

[8] Ren Nanqi, Chen Xiaolei, Zhao Dan. Control of fermentation types in continuous-flow acidogenic reactors: effects of pH and redox potential[J]. Journal of harbin institute of technology, 2001, 8(2): 116~119.

[9] Ren Nanqi, Wang Banzhen. Study of Carbohydrate to Ethanol-type Fermentation in Efficiency Acidogenic Reactor. [J]. Biotechnology 1997, 54(5): 428~433.

DOI: 10.1002/(sici)1097-0290(19970605)54:5<428::aid-bit3>3.0.co;2-g

[10] LIN Ming , REN Nan-qi , WANG Ai-jie , ZHANG Ying , WANG Xiang-jing , MA Fang, Promotion of hydrogen producing ability of hydrogen producing bacteria by several kinds of metal ions[J], Journal of Harbin Institute of Technology, 2003, 35(2): 147~151.

[11] State Environmental Protection Administration (SEPA), Water and Wastewater Monitoring Analysis Method(Ⅳ)(M) China Environment Science Press 2006, 11: 102~210.