In Silico Cloning of the TIR1 cDNA from Zea Mays and Bioinformatic Analysis
Based on the data of HTGS database and Blast between Zea Mays genome and Oryza Sativa TIR1 gene, putative Zea Mays TIR1 gene of a 3150 bp - length cDNA sequence with an full ORF was obtained. The gene shared high similarity with other plant TIR1 genes, indicating that it might be a new member of TIR1 family. On this base, through public database and related softwares, the primary structure of the protein Zea Mays TIR1 such as physical and chemical characterization was analyzed, and then the secondary and tertiary structure was emulated. We also structured Phylogenetic tree of some plant TIR1. The above resultsmay play an important role in the further research for isolatiion, identification and application of Zea Mays TIR1 gene.
Q. D. Meng and J. Y. Du, "In Silico Cloning of the TIR1 cDNA from Zea Mays and Bioinformatic Analysis", Advanced Materials Research, Vol. 159, pp. 656-661, 2011