The objective of this study is to assess the potential applicability of an extracellular biopolymer (PA-2) produced by Pseudomonas alcaligenes as biosorbent remove the Cu(II) ions from environmental and industrial wastewater. The effects such as pH, Cu(II) initial concentration and sorbate-sorbent contact time and agitating speed on the adsorption capacities of PA-2 were studied. Biosorption equilibriums were rapidly established in about 60 min and the adsorption kinetics followed the pseudo-second order kinetic model. Biosorption equilibrium datas were better described by Langmuir isotherm model. The maximum Cu(II) adsorption capacity determined from Langmuir isotherm were 81.36 mg /g PA-2. The carboxyl and hydroxyl groups of the PA-2 were involved in chemical interaction with the Cu(II) ions depicted by Fourier transform infrared spectroscopic (FTIR) results. The present study indicated that PA-2 may be used as an inexpensive, effective and easily cultivable biosorbent for the removal of Cu(II) ions from environmental and industrial wastewater.