The Study of Existential State of Protein in Protein/Montmorillonite Complexes

Abstract:

The existential state of protein in complexes directly affects the performance and applications of the composite materials. The interlayer space changes of montmorillonite in the protein / montmorillonite (MMT) composite were identified by X-ray diffraction (XRD). And the interaction between protein and MMT were analyzed by Fourier transform infrared spectrometry (FT-IR) and UV/vis spectrophotometry. The loading amount of bovine serum albumin (BSA) onto MMT was calculated from the TG data. The types of adsorption isotherm of BSA onto montmorillonite were analyzed. From the above analysis, it can be concluded that the structure of proteins in the montmorillonite interlayers has been changed, and the hydrogen bond and Van der Waals force between the BSA molecules and montmorillonite crystal layers are intensified. The α-helix content of BSA molecules reduces while random coil increases. The protein shows a state of being squashed.

• Periodical: Advanced Materials Research (Volumes 194-196)
• Pages: 1647-1651
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.194-196.1647
• Online since: February 2011
• Authors: Ying Hai Lv, Gui Jiang Li, Li Qiang Cui, Hua Xiao Yan, Shi Xue Zhou
• Keywords: Complexe, Existential State, Montmorillonite, Protein

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