The Use of CARD-FISH to Evaluate the Quantitative Microbial Ecology Involved in the Continuous Bioleaching of a Cobaltiferrous Pyrite

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Catalyzed reporter deposition fluorescence in situ hybridization (CARD-FISH) is a powerful method with a growing number of applications in the quantitative evaluation of microbial populations of complex ecosystems. CARD-FISH is an improvement over traditional fluorescence in situ hybridization (FISH) especially suitable for aquatic habitats with small, slow growing, or starving bacteria, in which the signal intensities of hybridized cells is frequently below detection limits or lost in high fluorescence background of dense mineral matrixes. In this work we report the development of protocols and probes for the identification and quantification of the microorganisms involved in the continuous bioleaching of a cobaltiferrous concentrate using a four tank-leaching reactor operated by BRGM. After steady state was reached, samples were taken to identify and quantify the microorganisms present in the each of the tanks used in the process.

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Periodical:

Advanced Materials Research (Volumes 20-21)

Edited by:

Axel Schippers, Wolfgang Sand, Franz Glombitza and Sabine Willscher

Pages:

565-568

Citation:

E. Díaz et al., "The Use of CARD-FISH to Evaluate the Quantitative Microbial Ecology Involved in the Continuous Bioleaching of a Cobaltiferrous Pyrite", Advanced Materials Research, Vols. 20-21, pp. 565-568, 2007

Online since:

July 2007

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[10] [20] [30] [40] [50] [60] [70] [80] [90] Feed R1 R2 R3 Continuous Bioleaching reactors Cells detected (%) EUB33 NTR12 GAM42a LEP154 THIO1 SUL1238 FER656+TMP65454 100.