The Use of CARD-FISH to Evaluate the Quantitative Microbial Ecology Involved in the Continuous Bioleaching of a Cobaltiferrous Pyrite

E. Díaz; E. González-Toril; Catherine Joulian; R. Amils

doi:10.4028/www.scientific.net/AMR.20-21.565

Paper Titles

Isolation and Molecular Characterization of Sulfate Reducing Bacteria in a Brazilian Acid Mine Drainage
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C-di-GMP Pathway in Acidithiobacillus ferrooxidans: Analysis of Putative Diguanylate Cyclases (DGCs) and Phosphodiesterases (PDEs) Bifunctional Proteins
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Searching for Useful Bacteria on Chalcopyrite Leaching from Japanese Abandoned Mines
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Comparative Bioreduction of Fe(III) with Geobacter metallireducens and Bacillus infernus
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The Use of CARD-FISH to Evaluate the Quantitative Microbial Ecology Involved in the Continuous Bioleaching of a Cobaltiferrous Pyrite
p.565

The Effect of Multiple Stresses on Sulfolobus-like Cultures in Bioleaching Systems: Super-Imposing Stress Responses to Metal Concentration and Solids Loading
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A New Acid-Stable, Fe-Oxidizing/O₂-Reducing Supercomplex Spanning Both Inner and Outer Membranes, Isolated from the Extremophile Acidithiobacillus
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Transporter Protein Genes are Up-Regulated by Covellite in Acidithiobacillus thiooxidans
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Up- and Down-Regulated Genes in Acidithiobacillus ferrooxidans Exposed to Chalcopyrite
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HomeAdvanced Materials ResearchAdvanced Materials Research Vols. 20-21The Use of CARD-FISH to Evaluate the Quantitative...

The Use of CARD-FISH to Evaluate the Quantitative Microbial Ecology Involved in the Continuous Bioleaching of a Cobaltiferrous Pyrite

Abstract:

Catalyzed reporter deposition fluorescence in situ hybridization (CARD-FISH) is a powerful method with a growing number of applications in the quantitative evaluation of microbial populations of complex ecosystems. CARD-FISH is an improvement over traditional fluorescence in situ hybridization (FISH) especially suitable for aquatic habitats with small, slow growing, or starving bacteria, in which the signal intensities of hybridized cells is frequently below detection limits or lost in high fluorescence background of dense mineral matrixes. In this work we report the development of protocols and probes for the identification and quantification of the microorganisms involved in the continuous bioleaching of a cobaltiferrous concentrate using a four tank-leaching reactor operated by BRGM. After steady state was reached, samples were taken to identify and quantify the microorganisms present in the each of the tanks used in the process.

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Periodical:

Advanced Materials Research (Volumes 20-21)

Pages:

565-568

DOI:

https://doi.org/10.4028/www.scientific.net/AMR.20-21.565

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Online since:

July 2007

Authors:

E. Díaz, E. González-Toril, Catherine Joulian, R. Amils

Keywords:

CARD-FISH, Continuous Bioleaching, Fish, Quantitative Microbial Ecology

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