The method to remove natural organic matter in water at low tempreture of the status of the Huaihe River water was studied .Through 10t/h in a pilot scale, ultrasound-enhanced coagulation treatment was used to simulate the micro-polluted water. For the role of Ultrasound, different pharmaceutical dosage control、the control of different HRT ,select the optimal HRT and the dosage of drugs. Also investigated whether the design parameters is reasonable. The effectiveness of the tested coagulation was evaluated by measurements of turbidity, CODMn, UV254 and NH3-N.Results showed that: when accordance with 10t/h processing load operation, settled water turbidity is high, the removal of organic matter was poor results. However, when accordance with 8t/h processing load operation, PAC dosage of 5.4mg/l were added,The removal efficiency of turbidity, NH3-N, CODMn and UV254 were respectively 93.29%, 7.91%, 37% and 41.63%.On this basis, when open Ultrasonic mixer, the CODMn and UV254 removal rates were increased by 9.12% and 7.86% compared with conventional treatment (PAC 5.4 mg/L)but had little effect on the removal of ammonia.